breseq  version 0.25c  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 66,457 C→A 100% V32F (GTT→TTT)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase
RA 97,880 C→T 100% S265F (TCC→TTC)  murD → UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
JC 105,358 Δ3 bp 18.6% coding (54‑56/1152 nt) ftsZ → GTP‑binding tubulin‑like cell division protein
RA 167,632 C→A 100% P50Q (CCG→CAG)  fhuA → ferrichrome outer membrane transporter
RA 274,547 A→G 100% intergenic (‑206/‑2) insH ← / → mmuP IS5 transposase and trans‑activator/S‑methylmethionine transporter
MC JC 274,733 Δ97,240 bp 100% [mmuP][mhpD] 90 genes
RA 547,694 A→G 100% intergenic (+123/‑1156) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain/hypothetical protein
RA 547,831 +G 100% intergenic (+260/‑1019) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain/hypothetical protein
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 569,996 G→C 100% intergenic (+345/‑120) ybcK → / → ybcL recombinase/kinase inhibitor
MC JC 654,214 Δ1,199 bp 100% insH insH
RA 749,354 C→A 100% P17P (CCG→CCT ybgO ← fimbrial‑like adhesin protein
RA 758,300 G→A 9.9% G64S (GGT→AGT)  sdhB → succinate dehydrogenase, FeS subunit
RA 758,440 C→A 100% F110L (TTC→TTA sdhB → succinate dehydrogenase, FeS subunit
RA 758,450 T→A 100% Y114N (TAT→AAT)  sdhB → succinate dehydrogenase, FeS subunit
RA 887,234 C→A 100% A176A (GCG→GCT ybjJ ← transporter
RA 987,574 G→T 100% intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,093,686 T→C 100% V130A (GTA→GCA)  ycdT → diguanylate cyclase
RA 1,097,580 C→T 18.7% intergenic (+329/+226) ycdU → / ← serX inner membrane protein/tRNA‑Ser
RA 1,170,573 C→T 100% intergenic (‑164/‑77) ycfQ ← / → ycfR DNA‑binding transcriptional regulator/hypothetical protein
RA 1,197,797 C→T 41.5% H366H (CAC→CAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,809 C→T 38.6% T370T (ACC→ACT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,822 T→C 31.6% L375L (TTA→CTA)  icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,824 A→G 31.5% L375L (TTA→TTG icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,854 C→T 12.1% N385N (AAC→AAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,857 G→C 12.1% A386A (GCG→GCC icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,860 A→G 9.8% K387K (AAA→AAG icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,869 C→T 4.3% T390T (ACC→ACT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,213,001 T→C 4.0% intergenic (+245/+256) mcrA → / ← elbA 5‑methylcytosine‑specific restriction endonuclease B/hypothetical protein
RA 1,213,013 T→C 3.6% intergenic (+257/+244) mcrA → / ← elbA 5‑methylcytosine‑specific restriction endonuclease B/hypothetical protein
RA 1,213,026 C→T 2.1% intergenic (+270/+231) mcrA → / ← elbA 5‑methylcytosine‑specific restriction endonuclease B/hypothetical protein
RA 1,213,028 G→A 2.1% intergenic (+272/+229) mcrA → / ← elbA 5‑methylcytosine‑specific restriction endonuclease B/hypothetical protein
MC JC 1,301,041 Δ1,336 bp 100% insCinsD insC, insD
RA 1,318,876 G→A 100% P21S (CCT→TCT)  trpA ← tryptophan synthase subunit alpha
MC JC 1,413,639 Δ23,060 bp 100% [ydaO]ynaE 30 genes
RA 1,483,588 G→A 100% L322L (CTG→CTA ynbD → phosphatase, inner membrane protein
RA 1,496,775 Δ1 bp 100% coding (11/924 nt) ydcI ← DNA‑binding transcriptional regulator
RA 1,615,814 C→A 100% D202Y (GAC→TAC)  yneI ← aldehyde dehydrogenase
RA 1,669,599 Δ1 bp 100% coding (680/1221 nt) dgsA ← DNA‑binding transcriptional repressor
RA 1,844,073 G→A 100% intergenic (‑224/‑12) ynjH ← / → gdhA hypothetical protein/glutamate dehydrogenase, NADP‑specific
RA 1,907,281 C→A 0.79% intergenic (+308/‑121) yobD → / → yebN inner membrane protein/inner membrane protein
RA 1,918,872 T→A 100% S301T (TCC→ACC)  yebS → inner membrane protein
RA 1,926,746 G→A 2.1% intergenic (‑63/‑76) yobA ← / → holE hypothetical protein/DNA polymerase III, theta subunit
RA 1,973,476 G→A 100% L310L (CTC→CTT tar ← methyl‑accepting chemotaxis protein II
RA 2,005,401 C→T 100% E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,170,094 C→A 100% intergenic (‑437/+32) ogrK ← / ← yegR DNA‑binding transcriptional regulator/hypothetical protein
MC JC 2,320,795 Δ1,336 bp 100% insDinsC insD, insC
RA 2,406,075 C→T 100% V51I (GTT→ATT)  nuoF ← NADH:ubiquinone oxidoreductase, chain F
RA 2,416,102 G→A 100% L233L (CTG→TTG)  yfbS ← transporter
RA 2,538,628 C→T 100% T258T (ACC→ACT cysK → cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
JC 2,865,274 +46 bp 14.4% coding (817/876 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor
RA 2,866,111 A→G 100% intergenic (‑21/+159) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/outer membrane lipoprotein
RA 3,003,330 G→A 100% L371L (CTC→CTT ygeV ← DNA‑binding transcriptional regulator
RA 3,049,815 G→T 100% R387S (CGC→AGC)  visC ← oxidoreductase
RA 3,397,813 C→G 100% D141H (GAT→CAT)  yhdE ← hypothetical protein
RA 3,450,056 C→A 4.0% A426S (GCA→TCA)  rpoC ← RNA polymerase, beta prime subunit
MC JC 3,742,936 Δ1,336 bp 100% insDinsC insD, insC
MC JC 3,746,911 Δ1,199 bp 100% insH insH
RA 3,750,122 Δ1 bp 95.9% coding (365/1416 nt) tnaA ← tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,750,123 C→T 4.0% G122S (GGC→AGC)  tnaA ← tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
MC JC 3,750,591 Δ1,199 bp 100% insH insH
RA 3,898,657 C→G 100% L259V (CTG→GTG)  yiaJ → DNA‑binding transcriptional repressor
MC JC 3,958,123 Δ34 bp 100% coding (1156‑1189/1287 nt) dctA → C4‑dicarboxylic acid, orotate and citrate transporter
RA 4,090,749 A→T 100% Q135L (CAG→CTG)  malQ → 4‑alpha‑glucanotransferase
RA 4,165,991 A→C 100% K43T (AAA→ACA)  rpsL → 30S ribosomal protein S12
MC JC 4,310,363 Δ1,199 bp 100% insH[alsK] insH, [alsK]
RA 4,347,492 C→A 1.6% Q302K (CAG→AAG)  melA → alpha‑galactosidase
RA 4,371,271 Δ2 bp 100% intergenic (‑6/+299) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,546,535 T→A 100% intergenic (+296/‑182) fimB → / → fimE tyrosine recombinase/inversion of on/off regulator of fimA/tyrosine recombinase/inversion of on/off regulator of fimA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1288306–1288375 1288526–1288459 85–221 293 [289] [291] 295 ychS hypothetical protein
* * ÷ NC_007779 2176983 2178208 1226 460 [0] [0] 349 insH insH
* * ÷ NC_007779 3128819–3130002 3147473 17472–18655 293 [287] [0] 609 [yghO]–[yghZ] 19 genes
[yghO], insH, yghQ, yghR, yghS, yghT, pitB, gss, yghU, hybG, hybF, hybE, hybD, hybC, hybB, hybA, hybO, yghW, [yghZ]
* * ÷ NC_007779 3261134–3262332 3262337 6–1204 416 [0] [0] 308 insH–[tdcD] insH, [tdcD]

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 273179 =NA (NA)332 (0.940) 151/180 NT 97.4% coding (1109/1146 nt) ykfC hypothetical protein
?NC_007779 657877 = 9 (0.030)coding (164/210 nt) cspE DNA‑binding transcriptional repressor
* ? NC_007779 = 274373NA (NA)275 (0.780) 132/180 NT 96.8% intergenic (‑32/‑176) insH/mmuP IS5 transposase and trans‑activator/S‑methylmethionine transporter
?NC_007779 = 657880 9 (0.030)coding (167/210 nt) cspE DNA‑binding transcriptional repressor
* ? NC_007779 = 274373NA (NA)51 (0.140) 38/180 NT 12.9% intergenic (‑32/‑176) insH/mmuP IS5 transposase and trans‑activator/S‑methylmethionine transporter
?NC_007779 679371 = 345 (0.980)coding (458/555 nt) ybeT outer membrane protein
* ? NC_007779 289856 =NA (NA)290 (1.290) 106/114 NT 100% intergenic (‑327/+17) argF/insB ornithine carbamoyltransferase 2, chain F/IS1 transposase InsAB'
?NC_007779 = 2176982 0 (0.000)intergenic (‑281/‑69) gatB/insH PTS system galactitol‑specific transporter subunit IIB/IS5 element protein
* ? NC_007779 380484 =NA (NA)371 (1.050) 152/180 NT NA intergenic (‑418/‑46) yaiO/insC hypothetical protein/IS2 insertion element repressor InsA
?NC_007779 = 381407 NA (NA)coding (510/906 nt) insD IS2 insertion element transposase InsAB'
* ? NC_007779 381401 =NA (NA)365 (1.050) 143/176 NT NA coding (504/906 nt) insD IS2 insertion element transposase InsAB'
?NC_007779 = 381812 NA (NA)coding (303/387 nt) yaiF hypothetical protein
* ? NC_007779 = 608570NA (NA)350 (1.020) 135/174 NT 95.0% intergenic (+170/+112) insL/entD IS186/IS421 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
?NC_007779 1305025 = 19 (0.050)coding (413/921 nt) oppB oligopeptide transporter subunit
* ? NC_007779 1209367 =163 (0.460)40 (0.140) 34/148 NT 23.4% coding (290/630 nt) ycfK hypothetical protein
?NC_007779 1211196 = 128 (0.440)intergenic (‑500/‑66) tfaE/pin tail fiber assembly protein/site‑specific DNA recombinase
* ? NC_007779 = 1209382162 (0.460)38 (0.130) 34/148 NT 22.5% coding (305/630 nt) ycfK hypothetical protein
?NC_007779 = 1211179 128 (0.440)intergenic (‑483/‑83) tfaE/pin tail fiber assembly protein/site‑specific DNA recombinase
* ? NC_007779 1221489 =7 (0.020)316 (0.910) 135/176 NT 97.9% intergenic (+711/+2393) ymgF/ymgD hypothetical protein/hypothetical protein
?NC_007779 1301041 = NA (NA)intergenic (+219/‑89) ychE/insC inner membrane protein/IS2 element protein
* ? NC_007779 = 12214950 (0.000)291 (0.820) 132/180 NT 100% intergenic (+717/+2387) ymgF/ymgD hypothetical protein/hypothetical protein
?NC_007779 = 1302369 NA (NA)intergenic (+11/‑527) insD/oppA IS2 element protein/oligopeptide transporter subunit
* ? NC_007779 = 130503720 (0.060)394 (1.170) 144/172 NT 95.4% coding (425/921 nt) oppB oligopeptide transporter subunit
?NC_007779 2519723 = NA (NA)intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/transposase
* ? NC_007779 1397758 =NA (NA)300 (0.850) 130/180 NT 100% intergenic (‑122/‑32) ynaI/insH inner membrane protein/IS5 transposase and trans‑activator
?NC_007779 1672777 = 0 (0.000)intergenic (+111/‑313) ynfM/asr transporter/acid shock‑inducible periplasmic protein
* ? NC_007779 = 16727800 (0.000)298 (0.840) 136/180 NT 100% intergenic (+114/‑310) ynfM/asr transporter/acid shock‑inducible periplasmic protein
?NC_007779 1980209 = NA (NA)intergenic (‑289/+146) flhD/insH DNA‑binding transcriptional dual regulator/IS5 element protein
* ? NC_007779 1907280 =11 (0.030)344 (0.970) 148/180 NT 96.9% intergenic (+307/‑122) yobD/yebN inner membrane protein/inner membrane protein
?NC_007779 = 2412225 NA (NA)intergenic (‑55/‑782) insA/yfbQ IS1 element protein/aminotransferase
* ? NC_007779 = 190728818 (0.050)352 (0.990) 159/180 NT 95.1% intergenic (+315/‑114) yobD/yebN inner membrane protein/inner membrane protein
?NC_007779 2411458 = NA (NA)intergenic (‑147/+15) lrhA/insB DNA‑binding transcriptional repressor/IS1 element protein
* ? NC_007779 1980091 =0 (0.000)224 (0.630) 112/180 NT 100% intergenic (‑171/+264) flhD/insH DNA‑binding transcriptional dual regulator/IS5 element protein
?NC_007779 1980209 = NA (NA)intergenic (‑289/+146) flhD/insH DNA‑binding transcriptional dual regulator/IS5 element protein
* ? NC_007779 = 19800950 (0.000)342 (0.980) 139/178 NT 100% intergenic (‑175/+260) flhD/insH DNA‑binding transcriptional dual regulator/IS5 element protein
?NC_007779 2176984 = NA (NA)intergenic (‑283/‑67) gatB/insH PTS system galactitol‑specific transporter subunit IIB/IS5 element protein
* ? NC_007779 2178209 =0 (0.000)325 (0.920) 132/180 NT 100% intergenic (+178/+184) insH/gatZ IS5 element protein/D‑tagatose 1,6‑bisphosphate aldolase 2, subunit
?NC_007779 = 2412225 NA (NA)intergenic (‑55/‑782) insA/yfbQ IS1 element protein/aminotransferase
* ? NC_007779 2320794 =NA (NA)283 (0.800) 125/180 NT 100% intergenic (+633/+11) rcsB/insD DNA‑binding response regulator in two‑component regulatory system with RcsC and YojN/IS2 element protein
?NC_007779 = 2589834 0 (0.000)intergenic (+470/‑69) acrD/yffB aminoglycoside/multidrug efflux system/hypothetical protein
* ? NC_007779 = 2322124NA (NA)423 (1.200) 161/180 NT 100% intergenic (‑91/‑2589) insC/atoS IS2 element protein/sensory histidine kinase in two‑component regulatory system with AtoC
?NC_007779 2589830 = 0 (0.000)intergenic (+466/‑73) acrD/yffB aminoglycoside/multidrug efflux system/hypothetical protein
* ? NC_007779 2411458 =NA (NA)345 (0.980) 145/180 NT 94.3% intergenic (‑147/+15) lrhA/insB DNA‑binding transcriptional repressor/IS1 element protein
?NC_007779 = 2985055 21 (0.060)coding (553/1230 nt) yqeG transporter
* ? NC_007779 = 2412225NA (NA)282 (0.800) 134/180 NT 94.6% intergenic (‑55/‑782) insA/yfbQ IS1 element protein/aminotransferase
?NC_007779 2985047 = 16 (0.050)coding (545/1230 nt) yqeG transporter
* ? NC_007779 3147474 =0 (0.000)395 (1.120) 164/180 NT 100% coding (922/1041 nt) yghZ aldo‑keto reductase
?NC_007779 = 3262328 NA (NA)coding (1188/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic
* ? NC_007779 = 3186081NA (NA)351 (1.000) 145/178 NT 96.7% coding (26/2466 nt) yqiG outer membrane usher protein
?NC_007779 3512753 = 12 (0.030)coding (529/1026 nt) cytR DNA‑binding transcriptional dual regulator
* ? NC_007779 = 32611330 (0.000)374 (1.130) 153/168 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?NC_007779 = 3748109 NA (NA)intergenic (+150/+962) insH/tnaA IS5 element protein/tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_007779 3261134 =NA (NA)353 (1.000) 156/180 NT 99.7% intergenic (‑60/‑68) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?NC_007779 3265774 = 1 (0.000)coding (110/990 nt) tdcB catabolic threonine dehydratase, PLP‑dependent
* ? NC_007779 3261135 =NA (NA)294 (0.840) 130/178 NT 100% intergenic (‑61/‑67) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?NC_007779 3262338 = 0 (0.000)coding (1178/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic
* ? NC_007779 = 32657771 (0.000)396 (1.120) 161/180 NT 99.7% coding (107/990 nt) tdcB catabolic threonine dehydratase, PLP‑dependent
?NC_007779 3365411 = NA (NA)intergenic (+368/+146) yhcD/insH outer membrane protein/IS5 transposase and trans‑activator
* ? NC_007779 = 351275911 (0.030)331 (0.950) 133/178 NT 96.8% coding (535/1026 nt) cytR DNA‑binding transcriptional dual regulator
?NC_007779 = 3744262 NA (NA)intergenic (‑90/+31) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
* ? NC_007779 = 3744263NA (NA)343 (0.970) 146/180 NT 100% intergenic (‑91/+30) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
?NC_007779 3903274 = 0 (0.000)coding (37/825 nt) bax hypothetical protein
* ? NC_007779 3746911 =NA (NA)390 (1.100) 149/180 NT 100% intergenic (‑509/‑68) mdtL/insH multidrug efflux system protein/IS5 element protein
?NC_007779 = 3748646 0 (0.000)intergenic (+687/+425) insH/tnaA IS5 element protein/tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_007779 = 3748105NA (NA)356 (1.010) 147/180 NT 100% intergenic (+146/+966) insH/tnaA IS5 element protein/tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
?NC_007779 3748643 = 0 (0.000)intergenic (+684/+428) insH/tnaA IS5 element protein/tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_007779 = 39032797 (0.020)350 (1.000) 146/178 NT 98.1% coding (42/825 nt) bax hypothetical protein
?NC_007779 = 4504190 NA (NA)intergenic (+10/‑89) insD/yjgW IS2 insertion element transposase InsAB'/hypothetical protein
* ? NC_007779 3987186 =NA (NA)346 (0.990) 151/178 NT 100% intergenic (‑732/‑31) slp/insH outer membrane lipoprotein/IS5 transposase and trans‑activator
?NC_007779 = 4009203 0 (0.000)coding (1379/1623 nt) yhiJ hypothetical protein
* ? NC_007779 = 3988379NA (NA)348 (0.980) 143/180 NT 100% coding (746/783 nt) yhiS hypothetical protein
?NC_007779 4009199 = 0 (0.000)coding (1375/1623 nt) yhiJ hypothetical protein
* ? NC_007779 4310363 =NA (NA)350 (0.990) 153/180 NT 100% intergenic (‑287/‑68) yjcS/insH alkyl sulfatase/IS5 element protein
?NC_007779 4353540 = 0 (0.000)intergenic (‑118/+453) dcuB/dcuR C4‑dicarboxylate antiporter/DNA‑binding response regulator in two‑component regulatory system with DcuS
* ? NC_007779 = 4311557NA (NA)339 (0.960) 145/180 NT 100% coding (921/1029 nt) alsK D‑allose kinase
?NC_007779 = 4353543 0 (0.000)intergenic (‑121/+450) dcuB/dcuR C4‑dicarboxylate antiporter/DNA‑binding response regulator in two‑component regulatory system with DcuS
* ? NC_007779 4355060 =0 (0.000)375 (1.060) 152/180 NT 100% coding (1281/1632 nt) dcuS sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
?NC_007779 4502861 = NA (NA)intergenic (+241/‑46) intB/insC integrase/IS2 insertion element repressor InsA
* ? NC_007779 = 43550663 (0.010)365 (1.050) 152/176 NT 99.2% coding (1275/1632 nt) dcuS sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
?NC_007779 = 4504189 NA (NA)intergenic (+9/‑90) insD/yjgW IS2 insertion element transposase InsAB'/hypothetical protein
* ? NC_007779 4547362 =304 (0.860)28 (0.090) 23/162 NT 9.5% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?NC_007779 4547676 = 257 (0.810)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
* ? NC_007779 = 4547370306 (0.860)23 (0.070) 20/162 NT 8.0% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?NC_007779 = 4547666 257 (0.810)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin