Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 758,579 A→T 2.7% T157S (ACC→TCC)  sdhB → succinate dehydrogenase, FeS subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_007779758,5790A→T 2.7% 18.5 ‑0.3 ‑0.8332T157S (ACC→TCC) sdhBsuccinate dehydrogenase, FeS subunit
Reads supporting (aligned to +/- strand):  new base (5/4):  ref base (154/167):  total (159/173)
Fisher's exact test strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.65e-01

Only 100 of 344 total aligned reads displayed.

TAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGAC  >  NC_007779/758479‑758679
                                                                                                    |                                                                                                    
taatGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCaa                                                                                                      >  1:15916363/1‑101 (MQ=255)
    ggACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACctc                                                                                                  <  1:5865644/101‑1 (MQ=255)
    ggACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACctc                                                                                                  >  1:15772814/1‑101 (MQ=255)
     gACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACctct                                                                                                 <  1:10833720/101‑1 (MQ=255)
      aCAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCtt                                                                                                <  1:10050461/101‑1 (MQ=255)
      aCAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCtt                                                                                                <  1:13799870/101‑1 (MQ=255)
       cAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTg                                                                                               <  1:13371652/101‑1 (MQ=255)
       cAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTg                                                                                               <  1:15432618/101‑1 (MQ=255)
       cAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTg                                                                                               >  1:11989260/1‑101 (MQ=255)
           aTCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCg                                                                                           >  1:13520687/1‑101 (MQ=255)
            tCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGt                                                                                          <  1:3659030/101‑1 (MQ=255)
            tCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGt                                                                                          >  1:5467025/1‑101 (MQ=255)
            tCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGt                                                                                          <  1:7046002/101‑1 (MQ=255)
              cGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCt                                                                                        <  1:3420011/101‑1 (MQ=255)
              cGCCAGCTCGCGAGCATTTACAGATGCCAGAGAAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTAAACCTCTTGTCCGTCt                                                                                        >  1:13957490/1‑101 (MQ=255)
                ccAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCttt                                                                                      >  1:11523081/1‑101 (MQ=255)
                ccAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCttt                                                                                      <  1:1234584/101‑1 (MQ=255)
                ccAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGAATGTTGTTCAACCTCTTGTCCGTCttt                                                                                      <  1:15528886/101‑1 (MQ=255)
                caaGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCttt                                                                                      >  1:10154556/3‑101 (MQ=255)
                  aGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCt                                                                                    <  1:11245039/101‑1 (MQ=255)
                   gCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtg                                                                                   >  1:14161128/1‑101 (MQ=255)
                      cgcgAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtggtg                                                                                >  1:4595472/1‑101 (MQ=255)
                        cgAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGa                                                                              >  1:2905474/1‑101 (MQ=255)
                        cgAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGa                                                                              <  1:6825729/101‑1 (MQ=255)
                         gAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGaa                                                                             <  1:2029482/101‑1 (MQ=255)
                         gAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGaa                                                                             >  1:15669337/1‑101 (MQ=255)
                            cATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATcc                                                                          <  1:2979143/101‑1 (MQ=255)
                                 aCAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATa                                                                     >  1:537786/1‑101 (MQ=255)
                                  cAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCATCCTCTTGTCCGTCTTTCTGGTGGAATCCCGATaa                                                                    >  1:2801279/1‑101 (MQ=255)
                                     aTGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCACTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGtt                                                                 <  1:9362120/101‑1 (MQ=255)
                                       gCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTa                                                               >  1:6627409/1‑101 (MQ=255)
                                        ccAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTAt                                                              <  1:8820039/101‑1 (MQ=255)
                                          agagCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCg                                                            <  1:15125449/101‑1 (MQ=255)
                                           gagCAGCGCGAAAAACTCGACTGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCgg                                                           <  1:3944882/101‑1 (MQ=255)
                                           gagCAGCGCGAAAAACTCGACGGGCTGTATTAATGTATTCACTGCGCATGTTTTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCgg                                                           <  1:4368944/101‑1 (MQ=255)
                                           gagCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCgg                                                           <  1:1442360/101‑1 (MQ=255)
                                            agcagcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGc                                                          <  1:915711/101‑1 (MQ=255)
                                             gcagcGCGAAAAACTCGACGGGCTGTATGAATGTATTCACTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGcc                                                         >  1:507025/1‑101 (MQ=255)
                                               agcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCg                                                       >  1:4137952/1‑101 (MQ=255)
                                               agcGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCg                                                       >  1:7496063/1‑101 (MQ=255)
                                                 cgcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGc                                                     <  1:6452667/101‑1 (MQ=255)
                                                 cgcgAAAAACTCGACGGGCTGTATGAATGTATTCACTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGc                                                     <  1:4911935/101‑1 (MQ=255)
                                                  gcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCa                                                    >  1:10408719/1‑101 (MQ=255)
                                                  gcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCa                                                    >  1:5991926/1‑101 (MQ=255)
                                                  gcgAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCa                                                    <  1:9810698/101‑1 (MQ=255)
                                                      aaaaCTCGACGGGCTGTATGAATGTATTCACTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCt                                                >  1:4472028/1‑101 (MQ=255)
                                                       aaaCTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGTAGGCtt                                               <  1:1702854/101‑1 (MQ=255)
                                                           tCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTa                                           >  1:2221823/1‑101 (MQ=255)
                                                             gACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGc                                         >  1:8450449/1‑101 (MQ=255)
                                                              aCGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCg                                        >  1:6983662/1‑101 (MQ=255)
                                                              aCGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTCTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCg                                        <  1:13104567/101‑1 (MQ=255)
                                                               cGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATTGGCCCGGCAGGCTTGTTAGCgg                                       <  1:3204828/101‑1 (MQ=255)
                                                                gggCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGTAGGCTTGTTAGCGGc                                      >  1:11495181/1‑101 (MQ=255)
                                                                 ggCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCa                                     >  1:15559200/1‑101 (MQ=255)
                                                                 ggCTGTATGAATGTATTCTCTGCGCATGTTGTCAACCTCTTGGCCGGCTTTCTGGTGAATCCCGAGAAGTTTATCGGGCCCGGGGGCGTGTTataggggta                                   >  1:9524903/1‑93 (MQ=255)
                                                                  gCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCat                                    <  1:5764556/101‑1 (MQ=255)
                                                                  gCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCat                                    <  1:8670977/101‑1 (MQ=255)
                                                                    tGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCatat                                  >  1:7872841/1‑101 (MQ=255)
                                                                    tGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCatat                                  >  1:7319317/1‑101 (MQ=255)
                                                                    tGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCatat                                  >  1:7649308/1‑101 (MQ=255)
                                                                     gTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATc                                 <  1:7118343/101‑1 (MQ=255)
                                                                      tATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCg                                >  1:131074/1‑101 (MQ=255)
                                                                      tATGAATGTATTCTCTGCGAATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCg                                <  1:9717279/101‑1 (MQ=255)
                                                                         gAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTTGCGGCATATCGttt                             >  1:2730223/1‑101 (MQ=255)
                                                                         gAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGttt                             >  1:9289215/1‑101 (MQ=255)
                                                                         gAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGttt                             <  1:4887025/101‑1 (MQ=255)
                                                                         gAATGTATTCACTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGttt                             <  1:12672467/101‑1 (MQ=255)
                                                                           aTGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGTAGGCTTGTTAGCGGCATATCGTTTcc                           <  1:10276351/101‑1 (MQ=255)
                                                                           aTGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTcc                           <  1:2529208/101‑1 (MQ=255)
                                                                            tGTATTCTCTGCGCATGTTGTTCATCCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCt                          <  1:3711924/101‑1 (MQ=255)
                                                                            tGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGTAGGCTTGTTAGCGGCATATCGTTTCCt                          <  1:5121078/101‑1 (MQ=255)
                                                                            tGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCt                          <  1:5830843/101‑1 (MQ=255)
                                                                             gTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGTCTTGTTAGCGGCATATCGTTTCCtg                         <  1:13910901/101‑1 (MQ=255)
                                                                                  ctctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattga                    <  1:1344989/101‑1 (MQ=255)
                                                                                   tctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat                   <  1:11111810/101‑1 (MQ=255)
                                                                                   tctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat                   >  1:14754637/1‑101 (MQ=255)
                                                                                   tctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCtgattgat                   >  1:1242071/1‑101 (MQ=255)
                                                                                   actGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATTTCGTTTCCtgattgat                   >  1:4694256/2‑101 (MQ=255)
                                                                                    ctGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATa                  >  1:14031789/1‑101 (MQ=255)
                                                                                     tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg                 <  1:10956018/101‑1 (MQ=255)
                                                                                     tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg                 <  1:5266440/101‑1 (MQ=255)
                                                                                     tGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAg                 <  1:8060441/101‑1 (MQ=255)
                                                                                      gcgcATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGc                <  1:6894415/101‑1 (MQ=255)
                                                                                      gcgcATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGc                >  1:14487368/1‑101 (MQ=255)
                                                                                       cgcATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGcc               >  1:12789304/1‑101 (MQ=255)
                                                                                         cATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGt             >  1:9191393/1‑101 (MQ=255)
                                                                                         cATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGt             <  1:231230/101‑1 (MQ=255)
                                                                                         cATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGt             <  1:7576103/101‑1 (MQ=255)
                                                                                           tgttgtTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGa           >  1:7696951/1‑101 (MQ=255)
                                                                                           tgttgtTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGa           <  1:1136600/101‑1 (MQ=255)
                                                                                            gttgttCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGTAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGAt          <  1:14265454/101‑1 (MQ=255)
                                                                                            gttgttCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGAt          <  1:14671630/101‑1 (MQ=255)
                                                                                            gttgttCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGGGAt          >  1:3324251/1‑101 (MQ=255)
                                                                                             tttttCACCCTCTTGTCCTTTTTTCTGGGGGAACCCCAAAAAGTTTATCGCCCGGGCAGGTTTGTTAGCGGCATATCGTTCCCTGATTGAGAGCGGTAATa         <  1:12275064/98‑1 (MQ=255)
                                                                                             ttgttCAACCTCTTGTCCGTCTTTCTGGTGTAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATa         <  1:3549804/101‑1 (MQ=255)
                                                                                             ttgttCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGTAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATa         >  1:1812556/1‑101 (MQ=255)
                                                                                             ttgttCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGCCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATa         <  1:10583124/101‑1 (MQ=255)
                                                                                               gttCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCACGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATAcc       >  1:8972705/1‑101 (MQ=255)
                                                                                                 tCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGGTACCga     >  1:1171170/1‑101 (MQ=255)
                                                                                                  cAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCgag    >  1:13669040/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGGCCCGGCAGGCTTGTTAGCGGCATATCGTTTCCTGATTGATAGCCGTGATACCGAGAC  >  NC_007779/758479‑758679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.