Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 97,880 C→T 100% S265F (TCC→TTC)  murD → UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_00777997,8800C→T100.0% 1416.1 356S265F (TCC→TTC) murDUDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (178/178):  ref base (0/0):  total (178/178)

Only 100 of 358 total aligned reads displayed.

CGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACATTC  >  NC_007779/97782‑97977
                                                                                                  |                                                                                                 
cGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCg                                                                                                 <  1:14763805/101‑1 (MQ=255)
  tCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCggg                                                                                               <  1:4495669/101‑1 (MQ=255)
  tCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCggg                                                                                               <  1:4714828/101‑1 (MQ=255)
  tCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCggg                                                                                               <  1:23518/101‑1 (MQ=255)
   cAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGgc                                                                                              >  1:15191994/1‑101 (MQ=255)
   cAACATGGGTGACTATCACCTGAATCATCAGCAGGGCAAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGgc                                                                                              >  1:8418190/1‑101 (MQ=255)
    aaCATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGgca                                                                                             >  1:16066005/1‑101 (MQ=255)
     aCATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGgcag                                                                                            <  1:10881259/101‑1 (MQ=255)
      cATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGgcagc                                                                                           >  1:9281647/1‑101 (MQ=255)
       aTGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAACGTGCTGAATGTGAAAGAGATGAAACTTTTCGGgcagca                                                                                          >  1:9188621/1‑101 (MQ=255)
             gACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTa                                                                                    <  1:2641873/101‑1 (MQ=255)
             gACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTa                                                                                    <  1:1850917/101‑1 (MQ=255)
              aCTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTac                                                                                   >  1:15952238/1‑101 (MQ=255)
              aCTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTac                                                                                   >  1:2198763/1‑101 (MQ=255)
               cTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTaca                                                                                  <  1:12141343/101‑1 (MQ=255)
                tATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTacac                                                                                 >  1:7670460/1‑101 (MQ=255)
                 aTCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACAcc                                                                                >  1:16437618/1‑101 (MQ=255)
                 aTCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACAcc                                                                                >  1:7187529/1‑101 (MQ=255)
                  tCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCa                                                                               >  1:2761514/1‑101 (MQ=255)
                   cACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCaa                                                                              <  1:16608056/101‑1 (MQ=255)
                   cACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCaa                                                                              >  1:3304742/1‑101 (MQ=255)
                   cACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCaa                                                                              <  1:15838111/101‑1 (MQ=255)
                    aCCTGAATCATCAGCAGGACGAAGCCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAAt                                                                             <  1:11096302/101‑1 (MQ=255)
                     ccTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATg                                                                            <  1:18274818/101‑1 (MQ=255)
                       tGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcg                                                                          <  1:4868271/101‑1 (MQ=255)
                       tGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcg                                                                          >  1:10220856/1‑101 (MQ=255)
                         aaTCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgct                                                                        <  1:8256651/101‑1 (MQ=255)
                            catcaGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGCAAGAGATGAAACTTATCGGGCAGCATAACTACACCAATgcgctggc                                                                     >  1:13214899/1‑101 (MQ=255)
                            catcaGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggc                                                                     <  1:3463322/101‑1 (MQ=255)
                             atcaGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATtcgctggcg                                                                    >  1:17367086/1‑101 (MQ=255)
                             atcaGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcg                                                                    <  1:11116383/101‑1 (MQ=255)
                               cagcagGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggc                                                                  >  1:2487517/1‑101 (MQ=255)
                                agcagGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcg                                                                 >  1:14323671/1‑101 (MQ=255)
                                agcagGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcg                                                                 >  1:12948804/1‑101 (MQ=255)
                                 gcagGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcgc                                                                <  1:9545912/101‑1 (MQ=255)
                                  cagGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcgct                                                               >  1:17733421/1‑101 (MQ=255)
                                  cagGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcgct                                                               <  1:9359008/101‑1 (MQ=255)
                                   agGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcgctg                                                              <  1:4310494/101‑1 (MQ=255)
                                   agGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcgctg                                                              <  1:6573296/101‑1 (MQ=255)
                                    gggCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcgctgg                                                             <  1:5377592/101‑1 (MQ=255)
                                     ggCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcgctggc                                                            <  1:15536594/101‑1 (MQ=255)
                                     ggCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcgctggc                                                            <  1:18126504/101‑1 (MQ=255)
                                     ggCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATgcgctggcggcgctggc                                                            <  1:14573692/101‑1 (MQ=255)
                                       cGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCggcgctggcgc                                                          >  1:10988792/1‑101 (MQ=255)
                                        gAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCggcgctggcgct                                                         >  1:15007169/1‑101 (MQ=255)
                                         aaaCCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGgcgctggcgctg                                                        >  1:3167257/1‑101 (MQ=255)
                                         aaaCCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGACGgcgctggcgctg                                                        >  1:11569902/1‑101 (MQ=255)
                                          aaCCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGcgctggcgctgg                                                       <  1:1000982/101‑1 (MQ=255)
                                            ccTGGCTGCGGGTTAAAGGCCAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCa                                                     <  1:13389691/101‑1 (MQ=255)
                                             cTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAg                                                    <  1:7029493/101‑1 (MQ=255)
                                               ggCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAt                                                  <  1:11241305/101‑1 (MQ=255)
                                               ggCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAt                                                  >  1:16263188/1‑101 (MQ=255)
                                                gCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAtg                                                 >  1:8853086/1‑101 (MQ=255)
                                                  tGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAtgct                                               <  1:6448487/101‑1 (MQ=255)
                                                  tGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAtgct                                               >  1:17838426/1‑101 (MQ=255)
                                                  tGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAtgct                                               >  1:9493055/1‑101 (MQ=255)
                                                   gCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAtgctg                                              >  1:11416355/1‑101 (MQ=255)
                                                   gCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAtgctg                                              >  1:10517107/1‑101 (MQ=255)
                                                    cGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGAtgctgc                                             <  1:8175082/101‑1 (MQ=255)
                                                       gTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAgg                                          >  1:16906496/1‑101 (MQ=255)
                                                          aaaGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGtt                                       <  1:4937841/101‑1 (MQ=255)
                                                          aaaGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGAAGATGCTGCAGGGtt                                       >  1:2962069/1‑101 (MQ=255)
                                                           aaGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTa                                      <  1:11029083/101‑1 (MQ=255)
                                                            aGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAAATTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTAc                                     >  1:8313329/1‑101 (MQ=255)
                                                               cGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACcgc                                  <  1:1131706/101‑1 (MQ=255)
                                                               cGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACcgc                                  <  1:7341982/101‑1 (MQ=255)
                                                                gagaAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACcgcg                                 >  1:6766399/1‑101 (MQ=255)
                                                                  gaAAGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTg                               <  1:3823/101‑1 (MQ=255)
                                                                   aaaGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGc                              >  1:2000505/1‑101 (MQ=255)
                                                                   aaaGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGc                              >  1:1902752/1‑101 (MQ=255)
                                                                     aGTGCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCca                            >  1:5343920/1‑101 (MQ=255)
                                                                     aGTCCTCAATGTTAAAGAGATGAAACTTTTCGGGCAGCATAACCACACCAATTCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCca                            <  1:849459/101‑1 (MQ=255)
                                                                        gCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCcagca                         <  1:13760130/101‑1 (MQ=255)
                                                                        gCTGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCcagca                         >  1:14154580/1‑101 (MQ=255)
                                                                          tGAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCagcagc                       >  1:16805366/1‑101 (MQ=255)
                                                                           gAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGcc                      >  1:8700496/1‑101 (MQ=255)
                                                                           gAATGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGcc                      >  1:5787848/1‑101 (MQ=255)
                                                                             aTGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTg                    >  1:13539345/1‑101 (MQ=255)
                                                                             aTGTGAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTg                    <  1:17800481/101‑1 (MQ=255)
                                                                              tgtgAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGa                   >  1:7954280/1‑101 (MQ=255)
                                                                              tgtgAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGa                   <  1:12042346/101‑1 (MQ=255)
                                                                              tgtgAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGa                   <  1:14443523/101‑1 (MQ=255)
                                                                                tgAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGaaa                 <  1:3739377/101‑1 (MQ=255)
                                                                                 gAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAg                >  1:6229901/1‑101 (MQ=255)
                                                                                 gAAAGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAg                <  1:1651350/101‑1 (MQ=255)
                                                                                  aaaGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGc               <  1:9788005/101‑1 (MQ=255)
                                                                                   aaGAGATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCg              <  1:5967867/101‑1 (MQ=255)
                                                                                    agagATGAAACTTTTCGGGCAGCATAACTACACCAATGCTCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGt             <  1:3979457/101‑1 (MQ=255)
                                                                                    agagATGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGt             >  1:12745620/1‑101 (MQ=255)
                                                                                     gagaTGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCCGCAGCCTGAAAGCGtt            >  1:12453311/1‑101 (MQ=255)
                                                                                      agaTGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTa           >  1:5121743/1‑101 (MQ=255)
                                                                                       gaTGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTaa          >  1:13903084/1‑101 (MQ=255)
                                                                                       gaTGAAACTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTaa          >  1:16177257/1‑101 (MQ=255)
                                                                                           aaaCTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACcac      >  1:8444611/1‑101 (MQ=255)
                                                                                           aaaCTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACcac      >  1:12667592/1‑101 (MQ=255)
                                                                                            aaCTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACcaca     >  1:15406568/1‑101 (MQ=255)
                                                                                             aCTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACAt    >  1:11758399/1‑101 (MQ=255)
                                                                                             aCTTTTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACAt    <  1:10026996/101‑1 (MQ=255)
                                                                                               tttTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACATTc  >  1:17040546/1‑101 (MQ=255)
                                                                                               tttTCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACATTc  >  1:17218210/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGGCAGCATAACTACACCAATGCGCTGGCGGCGCTGGCGCTGGCAGATGCTGCAGGGTTACCGCGTGCCAGCAGCCTGAAAGCGTTAACCACATTC  >  NC_007779/97782‑97977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.