Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_007779	57,026	Δ21 bp	4.8%	coding (64‑84/2355 nt)	imp ←	exported protein required for envelope biosynthesis and integrity

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_007779	= 57025	233 (0.820)	12 (0.040)	12/180	NT	4.8%	coding (85/2355 nt)	imp	exported protein required for envelope biosynthesis and integrity
	?	NC_007779	57047 =	238 (0.840)					coding (63/2355 nt)	imp	exported protein required for envelope biosynthesis and integrity

GTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_007779/56936‑57025 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTT  >  NC_007779/57047‑57126                                                                                                                                                                            GTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGACTGTTGACTAT                                                                       >  1:8851059/1‑101       AGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGACTGTTGACTATAAAGGG                                                                 >  1:7153155/1‑101          ATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGACTGTTGACTATAAAGGGCGG                                                              >  1:3821936/1‑101                       GGTAAGTCATTGGTATCGCCCTGTCCCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGCCTGTTGACTATAAAGGGCGGTGGCAATCATGGT                                                 <  1:5600986/101‑1                           AGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGACTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCC                                             <  1:8866521/101‑1                                TTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGACTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAG                                        <  1:8252465/101‑1                                 TGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGACTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGA                                       <  1:8020413/101‑1                                                CCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGACTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTT                        >  1:2249821/1‑101                                                      GACGGTCATAGCTTGGCACGCCCAACATGCACTGTGACTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATA                  <  1:4528257/101‑1                                                          GTCATAGCTTGGCACGCCCAACATGCACTGTGACTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTT              <  1:6799679/101‑1                                                                   TGGCACGCCCAACATGCACTGTGACTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATT     <  1:12294066/101‑1                                                                      CACGCCCAACATGCACTGTGACTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTT  <  1:9284856/101‑1                                                                                                                                                                            GTGGTCAGCATTGATAGTCACGGGTAAGTCATTGGTATCGCCCTGTACCAGAGGACGGTCATAGCTTGGCACGCCCAACATGCACTGTGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_007779/56936‑57025 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGTTGACTATAAAGGGCGGTGGCAATCATGGTGGCCAGGAGAGTGGGGATACGTTTTTTCATACGTTGATTTTATTGTT  >  NC_007779/57047‑57126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.