mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.25c
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | 2_D28_AGTTCC_L003_R1_001 | 15,006,955 | 1,515,702,455 | 100.0% | 101.0 bases | 101 bases | 99.3% |
| total | 15,006,955 | 1,515,702,455 | 100.0% | 101.0 bases | 101 bases | 99.3% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_007779 | 4,646,332 | 323.2 | 2.5 | 100.0% | Escherichia coli str. K-12 substr. W3110, complete genome. |
| total | 4,646,332 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 100011 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 133 |
| Total length of all junction candidates (factor times the reference genome length) | ≥ 0.1 | 0.006 |
| reference sequence | pr(no read start) |
|---|---|
| NC_007779 | 0.25299 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | OFF |
| Polymorphism minimum coverage each strand | 2 |
| Polymorphism bias cutoff | 0.001 |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥2 bases |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 13:47:43 02 Mar 2015 | 13:51:10 02 Mar 2015 | 3 minutes 27 seconds |
| Read alignment to reference genome | 13:51:11 02 Mar 2015 | 14:03:01 02 Mar 2015 | 11 minutes 50 seconds |
| Preprocessing alignments for candidate junction identification | 14:03:01 02 Mar 2015 | 14:10:36 02 Mar 2015 | 7 minutes 35 seconds |
| Preliminary analysis of coverage distribution | 14:10:36 02 Mar 2015 | 14:24:14 02 Mar 2015 | 13 minutes 38 seconds |
| Identifying junction candidates | 14:24:14 02 Mar 2015 | 14:24:24 02 Mar 2015 | 10 seconds |
| Re-alignment to junction candidates | 14:24:24 02 Mar 2015 | 14:26:42 02 Mar 2015 | 2 minutes 18 seconds |
| Resolving alignments with junction candidates | 14:26:42 02 Mar 2015 | 14:38:18 02 Mar 2015 | 11 minutes 36 seconds |
| Creating BAM files | 14:38:18 02 Mar 2015 | 14:52:18 02 Mar 2015 | 14 minutes 0 seconds |
| Tabulating error counts | 14:52:18 02 Mar 2015 | 14:58:21 02 Mar 2015 | 6 minutes 3 seconds |
| Re-calibrating base error rates | 14:58:21 02 Mar 2015 | 14:58:23 02 Mar 2015 | 2 seconds |
| Examining read alignment evidence | 14:58:23 02 Mar 2015 | 18:18:32 02 Mar 2015 | 3 hours 20 minutes 9 seconds |
| Polymorphism statistics | 18:18:32 02 Mar 2015 | 18:18:34 02 Mar 2015 | 2 seconds |
| Output | 18:18:34 02 Mar 2015 | 18:21:13 02 Mar 2015 | 2 minutes 39 seconds |
| Total | 4 hours 33 minutes 29 seconds | ||
