Predicted mutation
evidence seq id position mutation freq annotation gene description
JC NC_007779 2,865,278 +TTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGC 51.1% coding (813/876 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 2865229 =120 (0.550)67 (0.580) 42/96 NT 51.1% coding (862/876 nt) rpoS RNA polymerase, sigma S (sigma 38) factor
?NC_007779 = 2865278 120 (0.550)coding (813/876 nt) rpoS RNA polymerase, sigma S (sigma 38) factor

GCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_007779/2865269‑2865229
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCG  <  NC_007779/2865278‑2865235
                                         ||||||||||||||||||||||||||||||||||||||||||                                            
GCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAAT                            <  1:11085037/101‑1
GCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAAT                            >  1:9484971/1‑101
 CGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATC                           <  1:910059/101‑1
 CGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATC                           <  1:146057/101‑1
  GCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCC                          <  1:13991512/101‑1
  GCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCC                          >  1:6100442/1‑101
  GCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCC                          <  1:1430968/101‑1
  GCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCC                          <  1:7466960/101‑1
  GCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCC                          <  1:11866104/101‑1
   CGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCT                         <  1:7649676/101‑1
   CGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCT                         >  1:7170769/1‑101
   CGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCT                         <  1:3588972/101‑1
    GAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTG                        >  1:2123859/1‑101
    GAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTG                        >  1:977028/1‑101
    GAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTG                        <  1:14191684/101‑1
    GAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTG                        <  1:5586860/101‑1
     AAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGC                       <  1:1969249/101‑1
     AAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGC                       >  1:4537022/1‑101
     AAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGC                       <  1:5066939/101‑1
     AAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGC                       >  1:5922824/1‑101
      AATCCTGCAAACGCATGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCA                      >  1:3129687/1‑101
      AATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCA                      <  1:6651142/101‑1
       ATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAA                     <  1:10673766/101‑1
        TCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAA                    >  1:9483477/1‑101
          CTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACG                  >  1:12034734/1‑101
           TGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGC                 >  1:6439403/1‑101
           TGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGC                 <  1:2447985/101‑1
            GCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCA                <  1:3653091/101‑1
            GCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCA                >  1:13774711/1‑101
             CAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAG               <  1:568200/101‑1
              AAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGG              >  1:6068840/1‑101
              AAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGG              >  1:3677536/1‑101
               AACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGG             <  1:11371186/101‑1
               AACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGG             <  1:11581937/101‑1
               AACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGG             <  1:11767759/101‑1
               AACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGG             >  1:4766632/1‑101
                ACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGG            <  1:6852677/101‑1
                ACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGG            <  1:9011809/101‑1
                 CGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGC           <  1:2157649/101‑1
                 CGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGC           <  1:1683670/101‑1
                  GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT          <  1:4689353/101‑1
                  GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT          >  1:13674868/1‑101
                  GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT          >  1:8142755/1‑101
                  GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT          >  1:13214737/1‑101
                  GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT          >  1:1099637/1‑101
                  GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT          <  1:7316658/101‑1
                  GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT          >  1:7225611/1‑101
                  GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT          <  1:6519295/101‑1
                   CAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTG         <  1:14239252/101‑1
                   CAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTG         >  1:5546596/1‑101
                   CAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTG         <  1:5981882/101‑1
                   CAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTG         >  1:12776579/1‑101
                    AGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGA        >  1:11971581/1‑101
                    AGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGA        <  1:9150558/101‑1
                     GGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAA       <  1:12125827/101‑1
                     GGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAA       <  1:14306212/101‑1
                     GGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAA       >  1:9975226/1‑101
                      GGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAAT      <  1:12986674/101‑1
                      GGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAAT      <  1:13116358/101‑1
                      GGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAAT      >  1:9029663/1‑101
                      GGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAAT      >  1:4676487/1‑101
                       GGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATA     <  1:1500658/101‑1
                        GCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATAT    <  1:7362064/101‑1
                        GCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATAT    >  1:8788537/1‑101
                         CTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATC   >  1:1216369/1‑101
                          TGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCG  >  1:12686142/1‑101
                          TGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCG  <  1:13241370/101‑1
                                         ||||||||||||||||||||||||||||||||||||||||||                                            
GCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_007779/2865269‑2865229
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCG  <  NC_007779/2865278‑2865235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.