Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 1,485,419 G→T 8.4% E196D (GAG→GAT)  hrpA → ATP‑dependent helicase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0077791,485,4190G→T 8.4% 46.3 ‑3.2 ‑0.2260E196D (GAG→GAT) hrpAATP‑dependent helicase
Reads supporting (aligned to +/- strand):  new base (17/5):  ref base (127/110):  total (145/115)
Fisher's exact test strand distribution p-value = 4.24e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.69e-01

Only 100 of 275 total aligned reads displayed.

TCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAG  >  NC_007779/1485319‑1485519
                                                                                                    |                                                                                                    
tCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAagag                                                                                                      <  1:6236081/101‑1 (MQ=255)
 ccAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGt                                                                                                     <  1:488950/101‑1 (MQ=255)
  cAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGtt                                                                                                    >  1:3110569/1‑101 (MQ=255)
   aGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTtg                                                                                                   >  1:9539460/1‑101 (MQ=255)
     cAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTtgct                                                                                                 >  1:11339144/1‑101 (MQ=255)
      aaGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTtgctg                                                                                                >  1:3801451/1‑101 (MQ=255)
      aaGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTtgctg                                                                                                >  1:4132329/1‑101 (MQ=255)
       aGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTtgctgc                                                                                               <  1:12698040/101‑1 (MQ=255)
        gACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGcc                                                                                              <  1:12325681/101‑1 (MQ=255)
          ccGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCcgc                                                                                            >  1:2665460/1‑101 (MQ=255)
          ccGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCcgc                                                                                            >  1:9023644/1‑101 (MQ=255)
           cGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCcgcg                                                                                           >  1:11234047/1‑101 (MQ=255)
              ctgttgcgtgaGTACGACACCATCATCATCGATGAGGCGCACGAACGCAGCCTCAACATCGACTTCCTGCTGGGATATTTAAAGCATATTCTGCCgcagcg                                                                                        >  1:12191235/11‑101 (MQ=255)
              ctgctgATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCgcggcg                                                                                        <  1:5047408/101‑1 (MQ=255)
              ctgctgATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCgcggcg                                                                                        <  1:94782/101‑1 (MQ=255)
                  tgATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCt                                                                                    >  1:10906640/1‑101 (MQ=255)
                  tgATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCt                                                                                    >  1:9781674/1‑101 (MQ=255)
                   gcgtgaGTACGACACCATCATCATCGATGAGGCGCACGAACGCAGCCTCAACATCGACTTCCTGCTGGGATATTTAAAGCATATTCTGCCGCAGCGCccag                                                                                   >  1:13927677/6‑99 (MQ=255)
                   gATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTg                                                                                   >  1:8183926/1‑101 (MQ=255)
                   gATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTg                                                                                   >  1:7159100/1‑101 (MQ=255)
                    aTGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGa                                                                                  <  1:5505349/101‑1 (MQ=255)
                    aTGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGa                                                                                  <  1:5860140/101‑1 (MQ=255)
                    aTGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGa                                                                                  <  1:10568080/101‑1 (MQ=255)
                       gaGTACGACACCATCATCATCGATGAGGCGCACGAACGCAGCCTCAACATCGACTTCCTGCTGGGATATTTAAAGCATATTCTGCCGCAGCGCCCAGATCt                                                                               >  1:3962923/2‑101 (MQ=255)
                           aCGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTaaaa                                                                           >  1:1030582/1‑101 (MQ=255)
                            cGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTaaaaa                                                                          >  1:13632801/1‑101 (MQ=255)
                            cGACACCATCATCATCGATGAGGCGCACGAACGCAGCCTCAACATCGACTTCCTGCTGGGATATTTAAAGCATATTCTGCCGCAGCGCCCAGATCTgaaga                                                                          >  1:6974424/1‑99 (MQ=255)
                             gACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAAt                                                                         <  1:11485035/101‑1 (MQ=255)
                             gACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAAt                                                                         >  1:79173/1‑101 (MQ=255)
                             gACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAAt                                                                         >  1:2246341/1‑101 (MQ=255)
                               cacTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCa                                                                       >  1:5800604/1‑101 (MQ=255)
                               cacTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCa                                                                       <  1:637486/101‑1 (MQ=255)
                               cacTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCa                                                                       <  1:8538206/101‑1 (MQ=255)
                                acTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCAt                                                                      <  1:6488273/101‑1 (MQ=255)
                                  caTCATCATCGATGAGGCGCACGAACGCAGCCTCAACATCGACTTCCTGCTGGGATATTTAAAGCATATTCTGCCGCAGCGCCCAGATCTGAAGATCATTa                                                                    >  1:13104788/2‑101 (MQ=255)
                                   aTCATCATCGATGAGGCGCACGAACGCAGCCTCAACATCGACTTCCTGCTGGGATATTTAAAGCATATTCTGCCGCAGCGCCCAGATCTGAAGATCATTAt                                                                   >  1:1256947/1‑101 (MQ=255)
                                    tCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATc                                                                  >  1:5961525/1‑101 (MQ=255)
                                        tattGACGAAGCGCACGAACGCGGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACtt                                                              <  1:6072944/101‑1 (MQ=255)
                                        tattGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACtt                                                              >  1:5178823/1‑101 (MQ=255)
                                        tattGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACtt                                                              >  1:6609772/1‑101 (MQ=255)
                                        catcgatgaggcgcACGAACGCAGCCTCAACATCGACTTCCTGCTGGGATATTTAAAGCATATTCTGCCGCAGCGCCCAGATCTGAAGATCATTATCACtt                                                              >  1:10732479/11‑101 (MQ=255)
                                         attGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTc                                                             >  1:560600/1‑101 (MQ=255)
                                          ttGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTcc                                                            <  1:8949986/101‑1 (MQ=255)
                                          ttGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTcc                                                            <  1:14010441/101‑1 (MQ=255)
                                          ttGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTcc                                                            <  1:13677196/101‑1 (MQ=255)
                                           tGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCcg                                                           <  1:1638159/101‑1 (MQ=255)
                                           tGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCcg                                                           <  1:14117746/101‑1 (MQ=255)
                                            gACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCcgc                                                          >  1:10629122/1‑101 (MQ=255)
                                            gACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCcgc                                                          >  1:539387/1‑101 (MQ=255)
                                             aCGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCcgcg                                                         <  1:9592658/101‑1 (MQ=255)
                                               gaggcgcACGAACGCAGCCTCAACATCGACTTCCTGCTGGGATATTTAAAGCATATTCTGCCGCAGCGCCCAGATCTGAAGATCATTATCACTTCGGCGAc                                                       <  1:9751445/98‑1 (MQ=255)
                                               gaggcgcACGAACGCAGCCTCAACATCGACTTCCTGCTGGGATATTTAAAGCATATTCTGCCGCAGCGCCCAGATCTGAAGATCATTATCACTTCGGCGAc                                                       >  1:3270229/4‑101 (MQ=255)
                                               gAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGAc                                                       >  1:9388258/1‑101 (MQ=255)
                                                 aGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTa                                                     >  1:10802823/1‑101 (MQ=255)
                                                  gcgcACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTAt                                                    <  1:7595305/101‑1 (MQ=255)
                                                   cgcACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATc                                                   >  1:12276750/1‑101 (MQ=255)
                                                       cGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGAcc                                               <  1:2928474/101‑1 (MQ=255)
                                                       cGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGAcc                                               <  1:7435543/101‑1 (MQ=255)
                                                        gAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGAccc                                              >  1:9671881/1‑101 (MQ=255)
                                                        gAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGAccc                                              >  1:8292949/1‑101 (MQ=255)
                                                             cAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAAc                                         <  1:4713421/101‑1 (MQ=255)
                                                                ccTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCt                                      <  1:14304889/101‑1 (MQ=255)
                                                                  tGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCttt                                    >  1:9716233/1‑101 (MQ=255)
                                                                  tGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCttt                                    <  1:10374987/101‑1 (MQ=255)
                                                                   gAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCtttt                                   <  1:6428553/101‑1 (MQ=255)
                                                                    aaTATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTc                                  >  1:4440450/1‑101 (MQ=255)
                                                                     atatCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTcg                                 >  1:13413851/1‑101 (MQ=255)
                                                                      tatCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATAACTTCCGCGACTATCGACCCGGAACGCTTTTcgc                                >  1:1961421/1‑101 (MQ=255)
                                                                        tCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCgcgc                              <  1:3739827/101‑1 (MQ=255)
                                                                         cGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGcc                             >  1:10999841/1‑101 (MQ=255)
                                                                         cGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGcc                             >  1:14196310/1‑101 (MQ=255)
                                                                          gATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCa                            <  1:2960507/101‑1 (MQ=255)
                                                                            ttttttGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACt                          >  1:8582899/1‑101 (MQ=255)
                                                                             tttttGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACtt                         <  1:1841823/101‑1 (MQ=255)
                                                                             tttttGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACtt                         >  1:7673133/1‑101 (MQ=255)
                                                                              ttttGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACttt                        >  1:1564368/1‑101 (MQ=255)
                                                                              ttttGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACttt                        >  1:8005085/1‑101 (MQ=255)
                                                                               tttGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTta                       <  1:8562717/101‑1 (MQ=255)
                                                                               tttGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTta                       <  1:6945046/101‑1 (MQ=255)
                                                                               tttGCTCGGCTATTCGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTta                       <  1:12495453/101‑1 (MQ=255)
                                                                                   cTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTtaataa                   >  1:9190163/1‑101 (MQ=255)
                                                                                   cTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTtaataa                   <  1:7036693/101‑1 (MQ=255)
                                                                                   cTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTtaataa                   <  1:8634134/101‑1 (MQ=255)
                                                                                    tCGGCTATTTGAAATAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTaataat                  <  1:10068282/101‑1 (MQ=255)
                                                                                     cGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATg                 <  1:8073841/101‑1 (MQ=255)
                                                                                     cGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATg                 >  1:3881668/1‑101 (MQ=255)
                                                                                      ggCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATgc                >  1:2412118/1‑101 (MQ=255)
                                                                                       gCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATgcg               >  1:12591515/1‑101 (MQ=255)
                                                                                         tATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGcc             <  1:7372442/101‑1 (MQ=255)
                                                                                         tATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGcc             >  1:11546201/1‑101 (MQ=255)
                                                                                            ttGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGat          <  1:11785814/101‑1 (MQ=255)
                                                                                            ttGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGat          <  1:4233423/101‑1 (MQ=255)
                                                                                             tGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGatt         <  1:7854173/101‑1 (MQ=255)
                                                                                             tGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGatt         >  1:7346299/1‑101 (MQ=255)
                                                                                              gAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGatta        <  1:1747482/101‑1 (MQ=255)
                                                                                              gAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGatta        >  1:8926495/1‑101 (MQ=255)
                                                                                              gAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGatta        >  1:2884222/1‑101 (MQ=255)
                                                                                              gAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGatta        <  1:7517254/101‑1 (MQ=255)
                                                                                                aaGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGattatt      <  1:10089895/101‑1 (MQ=255)
                                                                                                  gagTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGATTATTGa    <  1:4828316/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
TCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCACGAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGTCCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCACTTTAATAATGCGCCGATTATTGAAG  >  NC_007779/1485319‑1485519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.