Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 80,487 G→A 8.5% C126C (TGC→TGT)  leuC ← 3‑isopropylmalate isomerase subunit, dehydratase component

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_00777980,4870G→A 8.5% 39.4 ‑4.3 ‑0.1211C126C (TGC→TGT) leuC3‑isopropylmalate isomerase subunit, dehydratase component
Reads supporting (aligned to +/- strand):  new base (14/4):  ref base (90/103):  total (104/107)
Fisher's exact test strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.29e-01

Only 100 of 228 total aligned reads displayed.

GTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAA  >  NC_007779/80387‑80587
                                                                                                    |                                                                                                    
gTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTcgccg                                                                                                      <  1:6245285/101‑1 (MQ=255)
  ttCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCa                                                                                                    <  1:6837385/101‑1 (MQ=255)
   gcAGGGTCTGGGTAGCCAGAACGTGCTCAACTTCGGAAGTACCGATACCAAAGGCCATCGCGCCGAAAGCACCGTGCGTTGCAGTGTGGGAGTCACCACAg                                                                                                   <  1:931052/100‑1 (MQ=255)
    cAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGa                                                                                                  >  1:1129308/1‑101 (MQ=255)
     aGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGAc                                                                                                 <  1:4385480/101‑1 (MQ=255)
      gggTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACa                                                                                                >  1:13452903/1‑101 (MQ=255)
      gggTCTGGGTAGCCAGAACGTGCTCAACTTCGGAAGTACCGATACCAAAGGCCATCGCGCCGAAAGCACCGTGCGTTGCAGTGTGGGAGTCACCACAGACa                                                                                                >  1:14036633/1‑101 (MQ=255)
       ggTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACaa                                                                                               <  1:8191948/101‑1 (MQ=255)
       ggTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACaa                                                                                               <  1:694033/101‑1 (MQ=255)
        gtctgggtagCCAGAACGTGCTCAACTTCGGAAGTACCGATACCAAAGGCCATCGCGCCGAAAGCACCGTGCGTTGCAGTGTGGGAGTCACCACAGACaac                                                                                              >  1:9425320/10‑100 (MQ=255)
        gTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAAt                                                                                              <  1:2564309/101‑1 (MQ=255)
         tttGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATg                                                                                             >  1:4050627/1‑101 (MQ=255)
          ttGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATgg                                                                                            >  1:8068234/1‑101 (MQ=255)
          ttGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATgg                                                                                            <  1:6173862/101‑1 (MQ=255)
           tGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGt                                                                                           >  1:13140031/1‑101 (MQ=255)
              gTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCAt                                                                                        >  1:1161803/1‑101 (MQ=255)
               ttGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATc                                                                                       >  1:3450820/1‑101 (MQ=255)
                tGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATcc                                                                                      >  1:4488166/1‑101 (MQ=255)
                tGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATcc                                                                                      >  1:10654976/1‑101 (MQ=255)
                   cAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATACCCg                                                                                   <  1:8287203/101‑1 (MQ=255)
                    aGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCgg                                                                                  <  1:10905148/101‑1 (MQ=255)
                     gaaCGTGCTCAACTTCGGAAGTACCGATACCAAAGGCCATCGCGCCGAAAGCACCGTGCGTTGCAGTGTGGGAGTCACCACAGACAACGGTGGTGCCCggt                                                                                 >  1:4830807/3‑100 (MQ=255)
                       aCGTGCTCAACTTCGGAAGTACCGATACCAAAGGCCATCGCGCCGAAAGCACCGTGCGTTGCAGTGTGGGAGTCACCACAGACAACGGTGGTGCCCggttg                                                                               >  1:12666189/1‑98 (MQ=255)
                         gtgtTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAgg                                                                             <  1:14319115/101‑1 (MQ=255)
                          tgtTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGt                                                                            <  1:8914567/101‑1 (MQ=255)
                           gtTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTg                                                                           >  1:7547849/1‑101 (MQ=255)
                              cAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACg                                                                        <  1:12325634/101‑1 (MQ=255)
                              cAACTTCGGAAGTACCGATACCAAAGGCCATCGCGCCGAAAGCACCGTGCGTTGCAGTGTGGGAGTCACCACAGACAACGGTGGTGCCCggttgagtcagg                                                                        >  1:8549682/1‑91 (MQ=255)
                                aCTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGcc                                                                      <  1:3979920/101‑1 (MQ=255)
                                 cTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGccc                                                                     >  1:8003848/1‑101 (MQ=255)
                                  ttCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCt                                                                    <  1:7389472/101‑1 (MQ=255)
                                    cGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGt                                                                  >  1:1522550/1‑101 (MQ=255)
                                       aaGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTcc                                                               >  1:13362549/1‑101 (MQ=255)
                                       aaGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTcc                                                               >  1:4882328/1‑101 (MQ=255)
                                        aGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCg                                                              <  1:9898150/101‑1 (MQ=255)
                                        aGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCg                                                              >  1:980739/1‑101 (MQ=255)
                                           gCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGcc                                                           >  1:4504119/1‑101 (MQ=255)
                                           gCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGcc                                                           >  1:2157163/1‑101 (MQ=255)
                                            ccGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGccc                                                          <  1:6718306/101‑1 (MQ=255)
                                              gATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCa                                                        >  1:12122673/1‑101 (MQ=255)
                                                tACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCAtt                                                      >  1:2975491/1‑101 (MQ=255)
                                                 aCCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTa                                                     >  1:13270846/1‑101 (MQ=255)
                                                  ccAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTAc                                                    <  1:8403506/101‑1 (MQ=255)
                                                  ccAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTAc                                                    >  1:6108890/1‑101 (MQ=255)
                                                    agaGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGt                                                  <  1:11803823/99‑1 (MQ=255)
                                                    aaaGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGt                                                  >  1:11485054/1‑101 (MQ=255)
                                                    aaaGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGt                                                  >  1:12750913/1‑101 (MQ=255)
                                                    aaaGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGt                                                  >  1:5799661/1‑101 (MQ=255)
                                                       ggCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGa                                               >  1:4054161/1‑101 (MQ=255)
                                                         ccAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACg                                             <  1:10178060/101‑1 (MQ=255)
                                                         ccAGTGCGCCAAACGCGCCGTGGGTGGCAGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACg                                             <  1:11390923/101‑1 (MQ=255)
                                                           aGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGAt                                           >  1:4678762/1‑101 (MQ=255)
                                                           aGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGAt                                           >  1:4451901/1‑101 (MQ=255)
                                                            gTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATc                                          <  1:13686629/101‑1 (MQ=255)
                                                             tGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATcc                                         >  1:13741981/1‑101 (MQ=255)
                                                              gcgcCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATccc                                        <  1:2011419/101‑1 (MQ=255)
                                                                gcCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCGCCt                                      >  1:10122812/1‑101 (MQ=255)
                                                                gcCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCt                                      <  1:5254645/101‑1 (MQ=255)
                                                                 ccAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTg                                     <  1:647117/101‑1 (MQ=255)
                                                                 ccAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTg                                     >  1:8665460/1‑101 (MQ=255)
                                                                   aaaCGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGAt                                   <  1:709701/101‑1 (MQ=255)
                                                                   aaaCGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGAt                                   >  1:9252043/1‑101 (MQ=255)
                                                                   aaaCGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGAt                                   >  1:10016637/1‑101 (MQ=255)
                                                                    aaCGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATa                                  <  1:12585626/101‑1 (MQ=255)
                                                                     aCGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATAc                                 <  1:5224586/101‑1 (MQ=255)
                                                                     aCGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATAc                                 <  1:8165365/101‑1 (MQ=255)
                                                                       gcgcCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGATGATCCCCTGATACgg                               >  1:2337534/1‑101 (MQ=255)
                                                                       gcgcCGTGGGTGGCGGTATGCGAGTCGCAGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACgg                               <  1:5548646/101‑1 (MQ=255)
                                                                        cgcCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACggg                              >  1:12653826/1‑101 (MQ=255)
                                                                        cgcCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACggg                              <  1:881529/101‑1 (MQ=255)
                                                                          ccGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTg                            <  1:7713284/101‑1 (MQ=255)
                                                                           cGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGa                           <  1:5089024/101‑1 (MQ=255)
                                                                           cGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGa                           >  1:261140/1‑101 (MQ=255)
                                                                            gtgggtggCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGAt                          >  1:1764794/1‑101 (MQ=255)
                                                                            gtgggtggCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGAt                          >  1:1495582/1‑101 (MQ=255)
                                                                            gtgggtggCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGAt                          >  1:14111164/1‑101 (MQ=255)
                                                                             tgggtggCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGAtt                         >  1:11070299/1‑101 (MQ=255)
                                                                               ggtggCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCa                       >  1:10502452/1‑101 (MQ=255)
                                                                                gtggCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAg                      <  1:10354318/101‑1 (MQ=255)
                                                                                 tggCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAgg                     <  1:11928727/101‑1 (MQ=255)
                                                                                   gCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTc                   <  1:6278555/101‑1 (MQ=255)
                                                                                    cGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCa                  <  1:8983166/101‑1 (MQ=255)
                                                                                     ggTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCAt                 <  1:12415259/101‑1 (MQ=255)
                                                                                     ggTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCAt                 >  1:5943624/1‑101 (MQ=255)
                                                                                      gTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATa                >  1:13374928/1‑101 (MQ=255)
                                                                                         tGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAg             >  1:9664219/1‑101 (MQ=255)
                                                                                         tGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAg             <  1:13647880/101‑1 (MQ=255)
                                                                                           cGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGtt           >  1:7797382/1‑101 (MQ=255)
                                                                                           cGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGtt           <  1:6644762/101‑1 (MQ=255)
                                                                                           cGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGtt           >  1:3038890/1‑101 (MQ=255)
                                                                                            gAGTCGCCGCAGCCAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTc          <  1:3953227/101‑1 (MQ=255)
                                                                                              gTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGa        <  1:10955676/101‑1 (MQ=255)
                                                                                               tCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGAc       <  1:11126646/101‑1 (MQ=255)
                                                                                                  ccgcAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGcc    >  1:13957050/1‑101 (MQ=255)
                                                                                                  ccgcAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGcc    <  1:1560616/101‑1 (MQ=255)
                                                                                                  ccgcAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGcc    <  1:2083453/101‑1 (MQ=255)
                                                                                                  ccgcAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGcc    <  1:5150461/101‑1 (MQ=255)
                                                                                                   cgcAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCa   >  1:13024152/1‑101 (MQ=255)
                                                                                                   cgcAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCa   >  1:13824753/1‑101 (MQ=255)
                                                                                                   cgcAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCa   >  1:9127433/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAA  >  NC_007779/80387‑80587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.