Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 1,486,046 C→G 5.5% T405T (ACC→ACG)  hrpA → ATP‑dependent helicase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0077791,486,0460C→G 5.5% 30.0 ‑0.6 ‑0.3236T405T (ACC→ACG) hrpAATP‑dependent helicase
Reads supporting (aligned to +/- strand):  new base (8/5):  ref base (111/112):  total (119/117)
Fisher's exact test strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.65e-01

Only 100 of 252 total aligned reads displayed.

CGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGG  >  NC_007779/1485946‑1486146
                                                                                                    |                                                                                                    
 gTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCg                                                                                                     >  1:10151658/1‑101 (MQ=255)
   cTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGAt                                                                                                   <  1:11795020/101‑1 (MQ=255)
    tGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATc                                                                                                  >  1:2104095/1‑101 (MQ=255)
      ccAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCg                                                                                                >  1:12387556/1‑101 (MQ=255)
       cAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCgg                                                                                               >  1:13418972/1‑101 (MQ=255)
       cAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCgg                                                                                               <  1:10813725/101‑1 (MQ=255)
         aTCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGgag                                                                                             <  1:7910989/101‑1 (MQ=255)
           cAGCATAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGAt                                                                                           >  1:777788/1‑101 (MQ=255)
              cGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCt                                                                                        <  1:8252143/101‑1 (MQ=255)
              cGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCt                                                                                        <  1:3874089/101‑1 (MQ=255)
               gTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTg                                                                                       <  1:10503337/101‑1 (MQ=255)
                tAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGc                                                                                      >  1:5869418/1‑101 (MQ=255)
                tAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGc                                                                                      <  1:10084978/101‑1 (MQ=255)
                 aaaGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCg                                                                                     >  1:9390254/1‑101 (MQ=255)
                     gCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTAcc                                                                                 <  1:5266237/101‑1 (MQ=255)
                        gCTGTGGCCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAAc                                                                              <  1:11904872/101‑1 (MQ=255)
                          tgtgGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCt                                                                            >  1:11554676/1‑101 (MQ=255)
                          tgtgGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCt                                                                            >  1:6324069/1‑101 (MQ=255)
                           gtgGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTg                                                                           <  1:6548725/101‑1 (MQ=255)
                           gtgGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTg                                                                           >  1:1768259/1‑101 (MQ=255)
                           gtgGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTg                                                                           <  1:5895558/101‑1 (MQ=255)
                            tgGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTgg                                                                          >  1:3952803/1‑101 (MQ=255)
                              gTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGcc                                                                        <  1:11420091/101‑1 (MQ=255)
                               tCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCt                                                                       <  1:12559083/101‑1 (MQ=255)
                                cGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTc                                                                      >  1:12206868/1‑101 (MQ=255)
                                cGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTc                                                                      >  1:10603470/1‑101 (MQ=255)
                                   gtgtCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGt                                                                   <  1:12689702/101‑1 (MQ=255)
                                        cGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTAttt                                                              >  1:13801097/1‑101 (MQ=255)
                                        cGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTAttt                                                              >  1:10915019/1‑101 (MQ=255)
                                         gAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTAtttt                                                             <  1:7082115/101‑1 (MQ=255)
                                          aaGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGGTCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTg                                                            >  1:9761527/1‑101 (MQ=255)
                                          aaGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTg                                                            <  1:7858390/101‑1 (MQ=255)
                                           aGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGc                                                           <  1:1637994/101‑1 (MQ=255)
                                             ggATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAg                                                         <  1:8611089/101‑1 (MQ=255)
                                              gATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGa                                                        >  1:7044603/1‑101 (MQ=255)
                                                tCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATg                                                      <  1:9338692/101‑1 (MQ=255)
                                                tCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATg                                                      <  1:1499317/101‑1 (MQ=255)
                                                 ggcgaTTCGTCTCTACAGCGAGGAGGAATTTCTCTCTCGCCCCGAGTTTACGGATCCGGAGATTCTGCGCACCAATCTCGCTTCGGTTGTACTGCAAATGa                                                     <  1:10014330/97‑1 (MQ=255)
                                                 cTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGa                                                     <  1:13762662/101‑1 (MQ=255)
                                                  tGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATgcc                                                    >  1:3612980/1‑99 (MQ=255)
                                                  tGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGAc                                                    <  1:11591447/101‑1 (MQ=255)
                                                   gTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGAcc                                                   >  1:13246856/1‑101 (MQ=255)
                                                      ttCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACcgcg                                                <  1:12891114/101‑1 (MQ=255)
                                                      ttCGTCTCTACAGCGAGGAGGAATTTCTCTCTCGCCCCGAGTTTACGGATCCGGAGATTCTGCGCACCAATCTCGCTTCGGTTGTACTGCAAATGatttcg                                                >  1:3476298/1‑96 (MQ=255)
                                                          tctcTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctgg                                            <  1:6112989/101‑1 (MQ=255)
                                                          tctcTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctgg                                            >  1:13018117/1‑101 (MQ=255)
                                                           ctctATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggg                                           >  1:14066858/1‑101 (MQ=255)
                                                            tctATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctgggg                                          <  1:1989723/101‑1 (MQ=255)
                                                            tctATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctgggg                                          >  1:2950248/1‑101 (MQ=255)
                                                             ctATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggggc                                         >  1:12950007/1‑101 (MQ=255)
                                                              tATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggggct                                        >  1:1173250/1‑101 (MQ=255)
                                                              tATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggggct                                        >  1:9653584/1‑101 (MQ=255)
                                                               aTTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggggctg                                       <  1:4885331/101‑1 (MQ=255)
                                                               aTTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggggctg                                       >  1:2410198/1‑101 (MQ=255)
                                                                 tCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggggctggg                                     <  1:855572/101‑1 (MQ=255)
                                                                 tCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggggctggg                                     >  1:8185857/1‑101 (MQ=255)
                                                                  ccGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGc                                    >  1:12072624/1‑101 (MQ=255)
                                                                    gAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGa                                  >  1:10413038/1‑101 (MQ=255)
                                                                     aaGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGat                                 >  1:1116420/1‑101 (MQ=255)
                                                                        aCGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATc                              <  1:11658639/101‑1 (MQ=255)
                                                                        aCGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATc                              <  1:6076940/101‑1 (MQ=255)
                                                                         cggtttCCTCTCGCGCCCGGAGTTTACCGATGCGGAGATTCTGCGTTCCACCCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCg                             <  1:468353/98‑1 (MQ=255)
                                                                         cGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCg                             <  1:11098793/101‑1 (MQ=255)
                                                                         cGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCg                             >  1:924507/1‑101 (MQ=255)
                                                                         cGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCg                             <  1:5644436/101‑1 (MQ=255)
                                                                          gATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGc                            >  1:3145148/1‑101 (MQ=255)
                                                                          gATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGc                            >  1:2602254/1‑101 (MQ=255)
                                                                             ttCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGc                         >  1:9839192/1‑101 (MQ=255)
                                                                             ttCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGc                         <  1:13458025/101‑1 (MQ=255)
                                                                             ttCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGc                         >  1:3525874/1‑101 (MQ=255)
                                                                              tCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCg                        <  1:6446696/101‑1 (MQ=255)
                                                                              tCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCg                        >  1:14195623/1‑101 (MQ=255)
                                                                               ccTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGt                       >  1:9482083/1‑101 (MQ=255)
                                                                               ccTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGt                       <  1:4738072/101‑1 (MQ=255)
                                                                                ctctCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGtt                      <  1:7549896/101‑1 (MQ=255)
                                                                                   tcGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTccc                   <  1:13175505/101‑1 (MQ=255)
                                                                                   tcGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTccc                   >  1:8620304/1‑101 (MQ=255)
                                                                                   tcGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTccc                   >  1:7841152/1‑101 (MQ=255)
                                                                                    cgcgCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCg                  >  1:12506429/1‑101 (MQ=255)
                                                                                     gcgcCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGt                 <  1:4852037/101‑1 (MQ=255)
                                                                                     gcgcCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGt                 >  1:4169954/1‑101 (MQ=255)
                                                                                       gcCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGttt               <  1:12348925/101‑1 (MQ=255)
                                                                                        cccGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTg              <  1:4139595/101‑1 (MQ=255)
                                                                                          cGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTc            >  1:5813548/1‑101 (MQ=255)
                                                                                           ggAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCg           >  1:11452193/1‑101 (MQ=255)
                                                                                           ggAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCg           <  1:7189779/101‑1 (MQ=255)
                                                                                           ggAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGATCCCGTTTGTCg           <  1:2699975/101‑1 (MQ=255)
                                                                                            gAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGa          <  1:5510105/101‑1 (MQ=255)
                                                                                            gAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGa          <  1:5015250/101‑1 (MQ=255)
                                                                                            gAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGa          >  1:6885521/1‑101 (MQ=255)
                                                                                             aGTTTACGGATCCGGAGATTCTGCGCACCAATCTCGCTTCGGTTGTACTGCAAATGATTTCGTTGCGGCTTGGCGCGATTGAGGCCTTCCCCTTTGTccag         >  1:6141460/1‑98 (MQ=255)
                                                                                             aGTTTACGGATCCGGAGATTCTGCGCACCAATCTCGCTTCGGTTGTACTGCAAATGATTTCGTTGCGGCTTGGCGCGATTGAGGCCTTCCCCTTTGTccag         <  1:4598961/101‑4 (MQ=255)
                                                                                             aGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGaa         >  1:8489811/1‑101 (MQ=255)
                                                                                             aGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGaa         <  1:5742522/101‑1 (MQ=255)
                                                                                              gTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAg        <  1:3296198/101‑1 (MQ=255)
                                                                                               tttACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGc       >  1:7440749/1‑101 (MQ=255)
                                                                                                 tACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCAc     >  1:13043233/1‑101 (MQ=255)
                                                                                                   ccGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCg   >  1:3299523/1‑101 (MQ=255)
                                                                                                    cGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCgg  <  1:656566/101‑1 (MQ=255)
                                                                                                    cGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCgg  <  1:4452727/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGG  >  NC_007779/1485946‑1486146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.