Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 3,460,411 A→G 4.4% T109T (ACT→ACC)  tufB ← protein chain elongation factor EF‑Tu

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0077793,460,4110A→G 4.4% 12.9 ‑1.3 ‑0.2180T109T (ACT→ACC) tufBprotein chain elongation factor EF‑Tu
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (88/84):  total (90/90)
Fisher's exact test strand distribution p-value = 2.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.99e-01

Only 100 of 184 total aligned reads displayed.

TCGCATTTGTTCAGGAACACGATGATGTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACGTGTGCGTAGT  >  NC_007779/3460322‑3460511
                                                                                         |                                                                                                     
tCGCACTTGTTCAGTGCAACGAGGATGTACGGAACGCCAACCTGGCGAGCCAGGAGAACGTGCTCACGGGTCTGCGGCATCGGACCGTCGGTAGCGGCGAc                                                                                            <  1:1143822/101‑1 (MQ=0)
                         tgTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTgc                                                                   >  1:4565697/1‑101 (MQ=11)
                          gTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTgcg                                                                  >  1:12602202/1‑101 (MQ=31)
                          gTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTgcg                                                                  <  1:9470050/101‑1 (MQ=31)
                           tACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTgcgc                                                                 <  1:10032514/101‑1 (MQ=32)
                           tACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTgcgc                                                                 >  1:1806542/1‑101 (MQ=32)
                            aCGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgca                                                                >  1:13110885/1‑101 (MQ=32)
                            aCGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgca                                                                <  1:8697277/101‑1 (MQ=32)
                            aCGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgca                                                                <  1:6413615/101‑1 (MQ=32)
                               gAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgcagca                                                             >  1:10950076/1‑101 (MQ=32)
                               gAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgcagca                                                             >  1:2078554/1‑101 (MQ=32)
                               gAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgcagca                                                             <  1:9426567/101‑1 (MQ=32)
                                aaCGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCAc                                                            <  1:10023221/101‑1 (MQ=32)
                                aaCGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCAc                                                            >  1:4235574/1‑101 (MQ=32)
                                 aCGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCAcc                                                           <  1:6936451/101‑1 (MQ=32)
                                 aCGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCAcc                                                           >  1:3368645/1‑101 (MQ=32)
                                  cGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCg                                                          >  1:13579829/1‑101 (MQ=32)
                                  cGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCg                                                          <  1:9411427/101‑1 (MQ=32)
                                   gCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCgg                                                         >  1:10386443/1‑101 (MQ=32)
                                   gCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCgg                                                         <  1:8357784/101‑1 (MQ=32)
                                   gCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCgg                                                         <  1:1574196/101‑1 (MQ=32)
                                   gCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCgg                                                         >  1:8493388/1‑101 (MQ=32)
                                   gCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCgg                                                         >  1:1020597/1‑101 (MQ=32)
                                    ccTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGt                                                        <  1:6057938/101‑1 (MQ=32)
                                    ccTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGt                                                        >  1:3744013/1‑101 (MQ=32)
                                    ccTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGt                                                        >  1:13646556/1‑101 (MQ=32)
                                    ccTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGt                                                        <  1:12519179/101‑1 (MQ=32)
                                    ccTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGt                                                        >  1:13126109/1‑101 (MQ=32)
                                      tACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGa                                                      >  1:4902165/1‑101 (MQ=32)
                                      tACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGa                                                      >  1:12764770/1‑101 (MQ=32)
                                       aCCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGAt                                                     >  1:3065321/1‑101 (MQ=32)
                                        ccTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATc                                                    <  1:10563799/101‑1 (MQ=33)
                                          tGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCAt                                                  <  1:11082541/101‑1 (MQ=33)
                                           gacgacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATg                                                 <  1:11193682/101‑1 (MQ=33)
                                           gacgacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATg                                                 >  1:11700602/1‑101 (MQ=32)
                                            acgacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGt                                                >  1:580591/1‑101 (MQ=32)
                                            acgacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGt                                                <  1:224914/101‑1 (MQ=33)
                                            acgacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGt                                                >  1:1168585/1‑101 (MQ=32)
                                              gacCCAGCAGGATGTGCTCACGAGTTTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGttt                                              >  1:3540218/1‑101 (MQ=17)
                                              gacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGttt                                              >  1:402561/1‑101 (MQ=32)
                                               acCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGtttt                                             >  1:5652892/1‑101 (MQ=32)
                                               acCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGtttt                                             <  1:9234983/101‑1 (MQ=32)
                                                cccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGttttt                                            >  1:11317760/1‑101 (MQ=32)
                                                cccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGttttt                                            >  1:5285465/1‑101 (MQ=32)
                                                 ccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTa                                           <  1:400270/101‑1 (MQ=32)
                                                 ccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTa                                           <  1:2887885/101‑1 (MQ=32)
                                                 ccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTa                                           >  1:8691370/1‑101 (MQ=32)
                                                  cagcagGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTaa                                          >  1:857762/1‑101 (MQ=32)
                                                    gcagGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACa                                        <  1:10343354/101‑1 (MQ=33)
                                                     cagGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACAt                                       <  1:6865150/101‑1 (MQ=32)
                                                      agGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCCCCGGTGATCATGTTTTTAACATa                                      >  1:8911029/1‑101 (MQ=17)
                                                      agGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATa                                      >  1:3255761/1‑101 (MQ=32)
                                                      agGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATa                                      >  1:12032080/1‑101 (MQ=32)
                                                        gATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGt                                    >  1:4938408/1‑101 (MQ=32)
                                                        gATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGt                                    <  1:270685/101‑1 (MQ=33)
                                                        gATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGt                                    >  1:8738010/1‑101 (MQ=32)
                                                        gATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGt                                    >  1:1515418/1‑101 (MQ=32)
                                                        gATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGt                                    >  1:10679241/1‑101 (MQ=32)
                                                         acgtgCTCACGGGTCTGCGGCATCGGACCGTCGGTAGCGGCGACAACCAGAATCGCGCCGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTc                                   >  1:5192636/3‑101 (MQ=2)
                                                         acgtgCTCACGGGTCTGCGGCATCGGACCGTCGGTAGCGGCGACAACCAGAATCGCGCCGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTc                                   <  1:6163981/99‑1 (MQ=2)
                                                         aTGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc                                   <  1:9888633/101‑1 (MQ=33)
                                                          tgtgCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg                                  <  1:1256305/101‑1 (MQ=33)
                                                           gtgCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg                                 >  1:2546752/1‑101 (MQ=32)
                                                           gtgCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg                                 <  1:11589702/101‑1 (MQ=33)
                                                            tgCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGc                                >  1:13185107/1‑101 (MQ=32)
                                                            tgCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGc                                <  1:10357599/101‑1 (MQ=33)
                                                            tgCTCACGAGTCTGCGGCATCGGGCCGTCAGGCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGc                                >  1:4440856/1‑101 (MQ=17)
                                                             gCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCg                               <  1:4631685/101‑1 (MQ=33)
                                                             gCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCg                               <  1:7250441/101‑1 (MQ=32)
                                                             gCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCg                               >  1:11800580/1‑101 (MQ=32)
                                                              cTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGt                              >  1:12118134/1‑101 (MQ=32)
                                                                 aCGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcc                           >  1:5525466/1‑101 (MQ=32)
                                                                  cGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGccc                          >  1:6324205/1‑101 (MQ=32)
                                                                   gAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcccc                         <  1:7180255/101‑1 (MQ=33)
                                                                    aGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg                        <  1:9956199/101‑1 (MQ=32)
                                                                    aGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg                        >  1:10997662/1‑101 (MQ=32)
                                                                     gTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCgg                       <  1:7417908/101‑1 (MQ=32)
                                                                     gTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCACAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCgg                       <  1:1690314/101‑1 (MQ=17)
                                                                         gCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAg                  <  1:5607472/101‑1 (MQ=32)
                                                                         gCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAg                  <  1:8093933/101‑1 (MQ=32)
                                                                         gCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAg                  <  1:9264531/101‑1 (MQ=32)
                                                                           ggCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTc                >  1:14066220/1‑101 (MQ=32)
                                                                            gCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCt               >  1:11973234/1‑101 (MQ=32)
                                                                            gCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCt               <  1:12643781/101‑1 (MQ=32)
                                                                             cATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTa              >  1:9632320/1‑101 (MQ=32)
                                                                              aTCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTAc             >  1:2786718/1‑101 (MQ=32)
                                                                                cGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACgt           <  1:6668859/101‑1 (MQ=32)
                                                                                 gggCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACgtg          >  1:10560462/1‑101 (MQ=32)
                                                                                 gggCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACgtg          >  1:3866483/1‑101 (MQ=32)
                                                                                  ggCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACgtgt         >  1:567483/1‑101 (MQ=32)
                                                                                  ggCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACgtgt         <  1:9676453/101‑1 (MQ=32)
                                                                                   accGTCGGTAGCGGCGACAACCAGAATCGCGCCGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTCGGCGTGGCCCGG‑AGCGTCGACgtgag        >  1:1125233/2‑99 (MQ=2)
                                                                                    ccGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACGTGTGc       <  1:3651332/101‑1 (MQ=32)
                                                                                    ccGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACGTGTGc       <  1:5994236/101‑1 (MQ=32)
                                                                                      gTCGGTAGCGGCGACAACCAGAATCGCGCCGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTCGGCGTGGCCCGGAGC‑GTCGACGTGAGCGt     <  1:1930463/101‑1 (MQ=2)
                                                                                      gTCGGTAGCGGCGACAACCAGAATCGCGCCGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTCGGCGTGGCCCGGAGC‑GTCGACGTGAGCGt     <  1:6194021/101‑1 (MQ=2)
                                                                                      gTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACGTGTGCGt     >  1:451005/1‑101 (MQ=33)
                                                                                        cAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACGTGTGCGTAg   >  1:520318/1‑101 (MQ=33)
                                                                                        cAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACGTGTGCGTAg   <  1:6421575/101‑1 (MQ=32)
                                                                                         aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACGTGTGCGTAGt  >  1:14231211/1‑101 (MQ=33)
                                                                                         |                                                                                                     
TCGCATTTGTTCAGGAACACGATGATGTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGG‑GCAGTCTACGTGTGCGTAGT  >  NC_007779/3460322‑3460511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.