Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 457,336 A→G 7.8% K229K (AAA→AAG)  clpX → ATPase and‑specificity subunit of ClpX‑ClpP ATP‑dependent serine protease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_007779457,3360A→G 7.8% 46.2 ‑5.5 ‑0.0257K229K (AAA→AAG) clpXATPase and‑specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  new base (3/17):  ref base (115/122):  total (118/139)
Fisher's exact test strand distribution p-value = 4.24e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.41e-01

Only 100 of 270 total aligned reads displayed.

CGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAG  >  NC_007779/457236‑457436
                                                                                                    |                                                                                                    
cGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTaaa                                                                                                      >  1:4232623/1‑101 (MQ=255)
   ccATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACAt                                                                                                   >  1:2134118/1‑101 (MQ=255)
    cATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATc                                                                                                  >  1:13294404/1‑101 (MQ=255)
     aTTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATcc                                                                                                 <  1:2405929/101‑1 (MQ=255)
       caCCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCgc                                                                                               >  1:9543432/2‑101 (MQ=255)
        aCCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCgca                                                                                              <  1:14531662/101‑1 (MQ=255)
        aCCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCgca                                                                                              <  1:8857860/101‑1 (MQ=255)
         cccGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCgcag                                                                                             <  1:5088855/101‑1 (MQ=255)
          ccGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCgcagc                                                                                            >  1:12384673/1‑101 (MQ=255)
          ccGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCgcagc                                                                                            <  1:6021431/101‑1 (MQ=255)
            gagaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGcagcag                                                                                          >  1:8452242/1‑101 (MQ=255)
            gagaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGcagcag                                                                                          >  1:1721844/1‑101 (MQ=255)
            gagaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGcagcag                                                                                          >  1:10373204/1‑101 (MQ=255)
              gaCGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGa                                                                                        <  1:4731261/101‑1 (MQ=255)
                cGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAAt                                                                                      >  1:435619/1‑101 (MQ=255)
                cGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAAt                                                                                      <  1:10141328/101‑1 (MQ=255)
                 gTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAAtt                                                                                     >  1:11468024/1‑101 (MQ=255)
                   ttCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCt                                                                                   >  1:6176824/1‑101 (MQ=255)
                    tCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCtt                                                                                  >  1:1596012/1‑101 (MQ=255)
                     ccGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTg                                                                                 <  1:12150131/101‑1 (MQ=255)
                        gTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAg                                                                              >  1:1867051/1‑101 (MQ=255)
                        gTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAg                                                                              >  1:9017401/1‑101 (MQ=255)
                         tGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAgg                                                                             >  1:2193009/1‑101 (MQ=255)
                         tGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTACTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAgg                                                                             >  1:8189140/1‑101 (MQ=255)
                          gAAGGCGTACAGCAGGCACTGTTGAAACTGCTCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTTCAggg                                                                            >  1:7446859/1‑100 (MQ=255)
                            aGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTg                                                                          >  1:16557/1‑101 (MQ=255)
                            aGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTg                                                                          >  1:3849851/1‑101 (MQ=255)
                             ggCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGa                                                                         <  1:9052654/101‑1 (MQ=255)
                                gTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATAc                                                                      >  1:980187/1‑101 (MQ=255)
                                 tACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATAcc                                                                     <  1:6970321/101‑1 (MQ=255)
                                  aCAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACct                                                                    >  1:3400127/1‑101 (MQ=255)
                                   cagcagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACctc                                                                   <  1:4835317/101‑1 (MQ=255)
                                     gcagGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTa                                                                 >  1:12552824/1‑101 (MQ=255)
                                       agGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAg                                                               >  1:12102156/1‑101 (MQ=255)
                                        ggCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGa                                                              <  1:14761948/101‑1 (MQ=255)
                                        ggCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGa                                                              <  1:8708631/101‑1 (MQ=255)
                                           aCTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATcc                                                           >  1:1140289/1‑101 (MQ=255)
                                            cTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCt                                                          <  1:912414/101‑1 (MQ=255)
                                              gTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGt                                                        <  1:4414934/101‑1 (MQ=255)
                                                 gAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTa                                                     <  1:9390580/101‑1 (MQ=255)
                                                  aaaCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTAt                                                    <  1:10209317/101‑1 (MQ=255)
                                                  aaaCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTAt                                                    <  1:13760404/101‑1 (MQ=255)
                                                   aaCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTAtt                                                   <  1:8108845/101‑1 (MQ=255)
                                                   aaCTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTAtt                                                   <  1:13623833/101‑1 (MQ=255)
                                                     cTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTtg                                                 <  1:14907618/101‑1 (MQ=255)
                                                     cTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTtg                                                 <  1:14320763/101‑1 (MQ=255)
                                                      tGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTtgt                                                >  1:4937547/1‑101 (MQ=255)
                                                       gATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTtgtg                                               <  1:8262143/101‑1 (MQ=255)
                                                       gATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTtgtg                                               >  1:9348170/1‑101 (MQ=255)
                                                        aTCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTgg                                              <  1:11130348/101‑1 (MQ=255)
                                                          cGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCATAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCg                                            >  1:9625960/1‑101 (MQ=255)
                                                           gAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCgg                                           <  1:4151149/101‑1 (MQ=255)
                                                            aaGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGt                                          >  1:3501428/1‑101 (MQ=255)
                                                              ggTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGc                                        <  1:9671279/101‑1 (MQ=255)
                                                                tACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGt                                      <  1:11882360/101‑1 (MQ=255)
                                                                 aCGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGtt                                     <  1:5538790/101‑1 (MQ=255)
                                                                 aCGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGtt                                     >  1:6462514/1‑101 (MQ=255)
                                                                  cGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGttt                                    <  1:2025245/101‑1 (MQ=255)
                                                                   ggTAGCGGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTg                                   >  1:14683004/1‑101 (MQ=255)
                                                                    gTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGc                                  <  1:9900150/101‑1 (MQ=255)
                                                                    gTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGc                                  >  1:2992199/1‑101 (MQ=255)
                                                                     tAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGcc                                 >  1:3286440/1‑101 (MQ=255)
                                                                      aGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCg                                >  1:8928649/1‑101 (MQ=255)
                                                                      aGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCg                                <  1:4652620/101‑1 (MQ=255)
                                                                        ctgctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGt                              <  1:6865213/101‑1 (MQ=255)
                                                                         tgctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTc                             >  1:13504250/1‑101 (MQ=255)
                                                                         tgctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTc                             >  1:6212158/1‑101 (MQ=255)
                                                                         tgctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTc                             <  1:14845692/101‑1 (MQ=255)
                                                                           ctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTg                           >  1:13104090/1‑101 (MQ=255)
                                                                           ctgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTg                           <  1:13485901/101‑1 (MQ=255)
                                                                            tgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTgg                          >  1:7801205/1‑101 (MQ=255)
                                                                            tgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTgg                          >  1:10083344/1‑101 (MQ=255)
                                                                            tgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTgg                          >  1:2769689/1‑101 (MQ=255)
                                                                            tgTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTACGTTTGCCGGTCTgg                          <  1:13868089/101‑1 (MQ=255)
                                                                             gtgcccccGCAGGGTGGGCGCAAGCTGCCGAACCAGGAGTTCATTCAGTTCGACACCTCCAATTTCCTGTTCATCGTTGCCGGTGCGTTTGCCGGTCTGGa                         >  1:10630528/7‑101 (MQ=255)
                                                                             gtgcccccGCAGGGTGGGCGCAAGCTGCCGAACCAGGAGTTCATTCAGTTCGACACCTCCAATGTCCTGTTCATCGTTGCCGGTGCGTTTGCCGGTCTGGa                         >  1:1874018/7‑101 (MQ=255)
                                                                             gTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGa                         >  1:13376556/1‑101 (MQ=255)
                                                                              tgcccccGCAGGGTGGGCGCAAGCTGCCGAACCAGGAGTTCATTCAGTTCGACACCTCCAATGTCCTGTTCATCGTTGCCGGTGCGTTTGCCGGTCTGGag                        <  1:15185876/96‑2 (MQ=255)
                                                                                ccACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATaa                      >  1:3026932/1‑101 (MQ=255)
                                                                                 ccccGCAGGGTGGGCGCAAGCTGCCGAACCAGGAGTTCATTCAGTTCGACACCTCCAATGTCCTGTTCATCGTTGCCGGTGCGTTTGCCGGTCTGGAGaag                     <  1:10657739/99‑2 (MQ=255)
                                                                                 ccccGCAGGGTGGGCGCAAGCTGCCGAACCAGGAGTTCATTCAGTTCGACACCTCCAATGTCCTGTTCATCGTTGCCGGTGCGTTTGCCGGTCTGGAGaag                     >  1:6735830/3‑100 (MQ=255)
                                                                                 ccccGCAGGGTGGGCGCAAGCTGCCGAACCAGGAGTTCATTCAGTTCGACACCTCCAATGTCCTGTTCATCGTTGCCGGTGCGTTTGCCGGTCTGGAGaag                     <  1:6517701/99‑2 (MQ=255)
                                                                                 cACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATaaa                     >  1:12665709/1‑101 (MQ=255)
                                                                                   ccGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGt                   <  1:5779475/101‑1 (MQ=255)
                                                                                    cGCAGGGTGGGCGCAAGCTGCCGAACCAGGAGTTCATTCAGTTCGACACCTCCAATGTCCTGTTCATCGTTGCCGGTGCGTTTGCCGGTCTGGAGAAGGtc                  <  1:6040348/101‑2 (MQ=255)
                                                                                    cGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTg                  <  1:11934767/101‑1 (MQ=255)
                                                                                     gCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGa                 <  1:372917/101‑1 (MQ=255)
                                                                                      cAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGAt                >  1:12939592/1‑101 (MQ=255)
                                                                                       agggTGGGCGCAAGCTGCCGAACCAGGAGTTCATTCAGTTCGACACCTCCAATGTCCTGTTCATCGTTGCCGGTGCGTTTGCCGGTCTGGAGAAGGtcgtg               <  1:13889061/99‑5 (MQ=255)
                                                                                       aaGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGAtt               >  1:5424843/1‑101 (MQ=255)
                                                                                        gggTGGGCGCAAGCTGCCGAACCAGGAGTTCATTCAGTTCGACACCTCCAATGTCCTGTTCATCGTTGCCGGTGCGTTTGCCGGTCTGGAGAAGGtcgtgc              <  1:14215303/100‑6 (MQ=255)
                                                                                             ggCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCAc         <  1:3937762/101‑1 (MQ=255)
                                                                                              gCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCAcc        <  1:11736060/101‑1 (MQ=255)
                                                                                               cGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCg       <  1:12014935/101‑1 (MQ=255)
                                                                                               cGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCg       <  1:11296495/101‑1 (MQ=255)
                                                                                                 tAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCgtg     <  1:1157017/101‑1 (MQ=255)
                                                                                                 tAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCgtg     >  1:10779551/1‑101 (MQ=255)
                                                                                                  aaaCATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCgtgt    >  1:8635708/1‑101 (MQ=255)
                                                                                                  aaaCATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCgtgt    >  1:4080847/1‑101 (MQ=255)
                                                                                                   aaCATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTa   <  1:10029781/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCCCACCGTGTAG  >  NC_007779/457236‑457436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.