Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 3,453,814 T→G 2.3% E541D (GAA→GAC)  rpoB ← RNA polymerase subunit beta

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0077793,453,8140T→G 2.3% 7.9 0.0 ‑0.2216E541D (GAA→GAC) rpoBRNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (114/97):  total (117/99)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.34e-01

Only 100 of 224 total aligned reads displayed.

GAGAGTTGATCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTGAGACAGCTGGCTGG  >  NC_007779/3453714‑3453914
                                                                                                    |                                                                                                    
 agagTTGATCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGtt                                                                                                     >  1:9816797/1‑101 (MQ=255)
 agagTTGATCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGtt                                                                                                     <  1:11151245/101‑1 (MQ=255)
 agagTTGATCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGtt                                                                                                     >  1:8482656/1‑101 (MQ=255)
  gagATGATCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTc                                                                                                    >  1:634/1‑101 (MQ=255)
    gTTGATCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCAc                                                                                                  <  1:15054349/101‑1 (MQ=255)
    gTTGATCAGACCGATGTTCGGACCTTCAGGCGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCAc                                                                                                  <  1:9068287/101‑1 (MQ=255)
      tGATCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACgg                                                                                                >  1:5756451/1‑101 (MQ=255)
      tGATCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACgg                                                                                                <  1:10972428/101‑1 (MQ=255)
        aTCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGt                                                                                              >  1:547454/1‑101 (MQ=255)
         tCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTc                                                                                             <  1:13853684/101‑1 (MQ=255)
         tCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTc                                                                                             >  1:3453624/1‑101 (MQ=255)
         tCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTc                                                                                             <  1:5335927/101‑1 (MQ=255)
         tCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTc                                                                                             >  1:7963927/1‑101 (MQ=255)
         tCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTAACGGGTc                                                                                             <  1:7736792/101‑1 (MQ=255)
         tCAGACCGATGTTCGGACCTGCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTc                                                                                             <  1:431993/101‑1 (MQ=255)
          cAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCa                                                                                            >  1:10021864/1‑101 (MQ=255)
          cAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCa                                                                                            >  1:1032256/1‑101 (MQ=255)
          cAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCa                                                                                            <  1:5724852/101‑1 (MQ=255)
          cAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCa                                                                                            <  1:8945926/101‑1 (MQ=255)
           aGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAg                                                                                           >  1:6418750/1‑101 (MQ=255)
           aGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAg                                                                                           <  1:9120964/101‑1 (MQ=255)
           aGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAg                                                                                           >  1:10373676/1‑101 (MQ=255)
             aCCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGAc                                                                                         >  1:9525559/1‑101 (MQ=255)
              ccGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGAcc                                                                                        <  1:6599936/101‑1 (MQ=255)
              ccGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGAcc                                                                                        <  1:10697807/101‑1 (MQ=255)
              ccGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGAcc                                                                                        <  1:7045817/101‑1 (MQ=255)
              ccGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCCCGAACTTCGAAGCCTGCACGTTCACGGGTCAGAcc                                                                                        <  1:10952604/101‑1 (MQ=255)
                gATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGc                                                                                      >  1:3612477/1‑101 (MQ=255)
                  tGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCt                                                                                    <  1:12223962/101‑1 (MQ=255)
                  tGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCt                                                                                    >  1:2825016/1‑101 (MQ=255)
                    ttCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTgg                                                                                  <  1:9885370/101‑1 (MQ=255)
                    ttCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTgg                                                                                  >  1:12958759/1‑101 (MQ=255)
                     tCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTggg                                                                                 <  1:5065565/101‑1 (MQ=255)
                     tCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTggg                                                                                 >  1:7119966/1‑101 (MQ=255)
                     tCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTggg                                                                                 <  1:1293445/101‑1 (MQ=255)
                     tCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTggg                                                                                 >  1:1267811/1‑101 (MQ=255)
                      cGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGc                                                                                >  1:2115708/1‑101 (MQ=255)
                       ggACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGcc                                                                               >  1:13322429/1‑101 (MQ=255)
                         aCCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGa                                                                             >  1:779098/1‑101 (MQ=255)
                           cTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGt                                                                           >  1:1476793/1‑101 (MQ=255)
                             tCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCTTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGc                                                                         <  1:12067209/101‑1 (MQ=255)
                              cAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCg                                                                        <  1:1186162/101‑1 (MQ=255)
                               aGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCgg                                                                       <  1:1377361/101‑1 (MQ=255)
                                    tttCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATa                                                                  >  1:11215674/1‑101 (MQ=255)
                                     ttCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATac                                                                 <  1:2261344/101‑1 (MQ=255)
                                      tCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATacg                                                                <  1:13743208/101‑1 (MQ=255)
                                       cGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATacga                                                               >  1:7851354/1‑101 (MQ=255)
                                        gATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATacgac                                                              >  1:8675067/1‑101 (MQ=255)
                                         aTTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATacgacg                                                             >  1:8970853/1‑101 (MQ=255)
                                           tGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGTTACGACGtt                                                           <  1:10125682/101‑1 (MQ=255)
                                               cATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTtgtg                                                       >  1:417461/1‑101 (MQ=255)
                                                 tACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCg                                                     <  1:2205530/101‑1 (MQ=255)
                                                   cgcgACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTa                                                   <  1:810471/101‑1 (MQ=255)
                                                    gcgACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTaa                                                  >  1:3590418/1‑101 (MQ=255)
                                                    gcgACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTaa                                                  >  1:6853795/1‑101 (MQ=255)
                                                      gACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAAtc                                                >  1:6167825/1‑101 (MQ=255)
                                                      gACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAAtc                                                >  1:4848873/1‑101 (MQ=255)
                                                         cGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCa                                             >  1:4136480/1‑101 (MQ=255)
                                                          gTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAg                                            >  1:5883231/1‑101 (MQ=255)
                                                           tAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGa                                           <  1:7653720/101‑1 (MQ=255)
                                                              tGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAg                                        <  1:4889554/101‑1 (MQ=255)
                                                              tGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAg                                        >  1:1838807/1‑101 (MQ=255)
                                                               gAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGc                                       >  1:2978790/1‑101 (MQ=255)
                                                               gAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGc                                       <  1:5583149/101‑1 (MQ=255)
                                                                aGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCg                                      <  1:14401981/101‑1 (MQ=255)
                                                                 gTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCgg                                     <  1:8847673/101‑1 (MQ=255)
                                                                   cGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGgt                                   <  1:10537395/101‑1 (MQ=255)
                                                                   cGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGgt                                   >  1:6051372/1‑101 (MQ=255)
                                                                    gggTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGCCAGCGGgtt                                  <  1:10114346/101‑1 (MQ=255)
                                                                    gggTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGgtt                                  >  1:286478/1‑101 (MQ=255)
                                                                    gggTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGgtt                                  >  1:13466552/1‑101 (MQ=255)
                                                                     ggTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGgttg                                 >  1:6354093/1‑101 (MQ=255)
                                                                     ggTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGgttg                                 <  1:6655922/101‑1 (MQ=255)
                                                                       tgtACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGgttgtt                               >  1:4688518/1‑101 (MQ=255)
                                                                         tACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCt                             <  1:14819113/101‑1 (MQ=255)
                                                                         tACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCt                             >  1:9585154/1‑101 (MQ=255)
                                                                          aCGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTg                            <  1:5947392/101‑1 (MQ=255)
                                                                           cGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTgg                           >  1:2385076/1‑101 (MQ=255)
                                                                           cGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTgg                           >  1:12193276/1‑101 (MQ=255)
                                                                            gTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGt                          <  1:3178268/101‑1 (MQ=255)
                                                                                cGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCAt                      <  1:12869212/101‑1 (MQ=255)
                                                                                cGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCAt                      >  1:15188425/1‑101 (MQ=255)
                                                                                 gAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATa                     <  1:14616724/101‑1 (MQ=255)
                                                                                  aaCTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATaa                    <  1:11740156/101‑1 (MQ=255)
                                                                                  aaCCTCGAGACCAGCACGGTCACGGGTCAGACCACCCGGACCCAGTGCGGACAGACGACGCTTGTGGGTCAGACCCGACAGCGAGTTGTTCTGGTCCAtga                    >  1:13636712/1‑99 (MQ=255)
                                                                                   aCTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATaaa                   >  1:564126/1‑101 (MQ=255)
                                                                                     ttCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACt                 <  1:8131630/101‑1 (MQ=255)
                                                                                      tCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTg                >  1:10794055/1‑101 (MQ=255)
                                                                                       cGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTga               <  1:10292260/101‑1 (MQ=255)
                                                                                       cGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTga               >  1:2575265/1‑101 (MQ=255)
                                                                                        gAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTgag              >  1:14560262/1‑101 (MQ=255)
                                                                                         aaGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTgaga             >  1:13003910/1‑101 (MQ=255)
                                                                                          aGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTGAGAc            <  1:6515558/101‑1 (MQ=255)
                                                                                              tGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTGAGACAgct        <  1:3916622/101‑1 (MQ=255)
                                                                                               gCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTGAGACAgctg       >  1:8191604/1‑101 (MQ=255)
                                                                                               gCACGGTCACGGGTCAGACCACCCGGACCCAGTGCGGACAGACGACGCTTGTGGGTCAGACCCGACAGCGAGTTGTTCTGGTCCATGAACTGGGACAgctg       <  1:7377106/101‑1 (MQ=255)
                                                                                                 aCGGTCACGGGTCAGACCACCCGGACCCAGTGCGGACAGACGACGCTTGTGGGTCAGACCCGACAGCGAGTTGTTCTGGTCCATGAACTGGGACAgctggg     >  1:1674763/1‑100 (MQ=255)
                                                                                                  cGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTGAGACAgctggct    >  1:5291610/1‑101 (MQ=255)
                                                                                                   gTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTGAGACAgctggctg   >  1:8245384/1‑101 (MQ=255)
                                                                                                   gTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTGAGACAgctggctg   <  1:7312318/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GAGAGTTGATCAGACCGATGTTCGGACCTTCAGGGGTTTCGATTGGACATACGCGACCGTAGTGAGTCGGGTGTACGTCTCGAACTTCGAAGCCTGCACGTTCACGGGTCAGACCGCCTGGGCCGAGTGCGGAGATACGACGTTTGTGCGTAATCTCAGACAGCGGGTTGTTCTGGTCCATAAACTGAGACAGCTGGCTGG  >  NC_007779/3453714‑3453914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.