Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_007779 3,460,447 C→A 4.1% A97A (GCG→GCT)  tufB ← protein chain elongation factor EF‑Tu

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0077793,460,4470C→A 4.1% 16.0 ‑1.2 ‑0.1221A97A (GCG→GCT) tufBprotein chain elongation factor EF‑Tu
Reads supporting (aligned to +/- strand):  new base (6/3):  ref base (98/114):  total (104/117)
Fisher's exact test strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.77e-01

Only 100 of 235 total aligned reads displayed.

TGTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTGAGAAGTGT  >  NC_007779/3460347‑3460547
                                                                                                    |                                                                                                    
tgTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTgc                                                                                                      <  1:428207/101‑1 (MQ=11)
tgTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTgc                                                                                                      >  1:9679954/1‑101 (MQ=11)
 gTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTgcg                                                                                                     <  1:662261/101‑1 (MQ=31)
  tACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTAGAGGCAACTACCAGGATCGCGCCGTCCATCTgcgc                                                                                                    >  1:3739002/1‑101 (MQ=255)
   aCGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgca                                                                                                   <  1:1034228/101‑1 (MQ=32)
    cGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgcag                                                                                                  >  1:6127416/1‑101 (MQ=32)
     ggAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgcagc                                                                                                 <  1:6388295/101‑1 (MQ=32)
     ggAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCgcagc                                                                                                 >  1:15092755/1‑101 (MQ=32)
        aCGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCAcc                                                                                              <  1:3022982/101‑1 (MQ=32)
        aCGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCAcc                                                                                              >  1:9495365/1‑101 (MQ=32)
         cGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCg                                                                                             <  1:11172436/101‑1 (MQ=32)
         cGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCg                                                                                             <  1:2012113/101‑1 (MQ=32)
          gCCTACCTGACGACCCAGCAGGATGTGCTCACTAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCgg                                                                                            >  1:3263980/1‑101 (MQ=17)
          gCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCgg                                                                                            <  1:14484204/101‑1 (MQ=32)
          gCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCgg                                                                                            >  1:8938309/1‑101 (MQ=32)
          gCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCgg                                                                                            >  1:11487550/1‑101 (MQ=32)
             tACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGa                                                                                         >  1:6074071/1‑101 (MQ=32)
             tACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGa                                                                                         >  1:12000894/1‑101 (MQ=32)
             tACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGa                                                                                         <  1:9437684/101‑1 (MQ=33)
                  gacgacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGCCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATg                                                                                    <  1:10622786/101‑1 (MQ=18)
                   acgacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGt                                                                                   <  1:5986335/101‑1 (MQ=33)
                   acgacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGt                                                                                   <  1:2937551/101‑1 (MQ=33)
                     gacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGttt                                                                                 <  1:10868237/101‑1 (MQ=33)
                     gacCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGttt                                                                                 >  1:1278905/1‑101 (MQ=32)
                      acCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGtttt                                                                                <  1:318599/101‑1 (MQ=32)
                        ccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTa                                                                              <  1:1688405/101‑1 (MQ=33)
                        ccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTa                                                                              >  1:7016538/1‑101 (MQ=32)
                        ccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTa                                                                              >  1:7471387/1‑101 (MQ=32)
                        ccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTa                                                                              >  1:10454001/1‑101 (MQ=32)
                        ccAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTa                                                                              >  1:13040716/1‑101 (MQ=32)
                            cagGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACAt                                                                          <  1:8056254/101‑1 (MQ=33)
                            cagGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACAt                                                                          <  1:4999919/101‑1 (MQ=33)
                             agGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATa                                                                         <  1:10510760/101‑1 (MQ=33)
                             agGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATa                                                                         <  1:14502087/101‑1 (MQ=33)
                              ggATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAg                                                                        <  1:13732539/101‑1 (MQ=33)
                              ggATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAg                                                                        <  1:1188775/101‑1 (MQ=32)
                               gATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGt                                                                       >  1:11300686/1‑101 (MQ=32)
                               gATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGt                                                                       >  1:4590741/1‑101 (MQ=32)
                                acgtgCTCACGGGTCTGCGGCATCGGACCGTCGGTAGCGGCGACAACCAGAATCGCGCCGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTc                                                                      >  1:5346072/3‑101 (MQ=2)
                                acgtgCTCACGGGTCTGCGGCATCGGACCGTCGGTAGCGGCGACAACCAGAATCGCGCCGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTc                                                                      <  1:233286/99‑1 (MQ=2)
                                acgtgCTCACGGGTCTGCGGCATCGGACCGTCGGTAGCGGCGACAACCAGAATCGCGCCGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTc                                                                      >  1:400660/3‑101 (MQ=2)
                                aTGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc                                                                      <  1:11602445/101‑1 (MQ=33)
                                aTGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc                                                                      >  1:13182191/1‑101 (MQ=32)
                                 tgtgCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg                                                                     <  1:9575330/101‑1 (MQ=33)
                                 tgtgCTCACGAGTCTGCGGCATCGGGCCGTCAGTCCCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg                                                                     >  1:10043065/1‑101 (MQ=17)
                                   tgCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGc                                                                   <  1:8326560/101‑1 (MQ=32)
                                   tgCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGc                                                                   >  1:11410720/1‑101 (MQ=32)
                                    gCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCg                                                                  >  1:6130574/1‑101 (MQ=32)
                                       cACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGc                                                               <  1:6159595/101‑1 (MQ=32)
                                       cACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGc                                                               >  1:6860663/1‑101 (MQ=32)
                                        aCGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcc                                                              <  1:8122115/101‑1 (MQ=32)
                                         cGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGccc                                                             >  1:2598949/1‑101 (MQ=32)
                                          gAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcccc                                                            <  1:1332199/101‑1 (MQ=32)
                                           aGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg                                                           <  1:12433306/101‑1 (MQ=33)
                                            gTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCgg                                                          >  1:11751609/1‑101 (MQ=32)
                                            gTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCgg                                                          <  1:1872781/101‑1 (MQ=32)
                                             tCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg                                                         >  1:2771725/1‑101 (MQ=32)
                                               tGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCa                                                       <  1:9336799/101‑1 (MQ=32)
                                                     aTCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc                                                 >  1:9726927/1‑101 (MQ=32)
                                                     aTCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc                                                 <  1:2760647/101‑1 (MQ=32)
                                                      tCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg                                                <  1:5574546/101‑1 (MQ=32)
                                                       cGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACgt                                               <  1:4547809/101‑1 (MQ=32)
                                                         ggCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACgtgt                                             >  1:7378539/1‑101 (MQ=33)
                                                           ccGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGc                                           <  1:4456261/101‑1 (MQ=32)
                                                             gTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt                                         <  1:1002508/101‑1 (MQ=32)
                                                              tCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTa                                        >  1:13555420/1‑101 (MQ=33)
                                                              tCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTa                                        <  1:1095505/101‑1 (MQ=32)
                                                               cAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAg                                       <  1:4869758/101‑1 (MQ=32)
                                                                aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt                                      <  1:4595543/101‑1 (MQ=32)
                                                                  tCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGa                                    >  1:7417998/1‑101 (MQ=33)
                                                                  tCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGa                                    <  1:9006840/101‑1 (MQ=32)
                                                                    gcagcaACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg                                  <  1:53836/101‑1 (MQ=32)
                                                                    gcagcaACTACCAGGATCGCGCCGTCCATCTGCCCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg                                  >  1:12134967/1‑101 (MQ=18)
                                                                     cagcaACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACgg                                 <  1:8262481/101‑1 (MQ=32)
                                                                        caACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTc                              <  1:646976/101‑1 (MQ=32)
                                                                        caACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTc                              <  1:3326360/101‑1 (MQ=32)
                                                                         aaCTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCg                             <  1:13254248/101‑1 (MQ=32)
                                                                         aaCTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGCTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGCAGTGACGGGTCg                             >  1:10742902/1‑101 (MQ=12)
                                                                            tACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCgggg                          >  1:10098362/1‑101 (MQ=33)
                                                                                aGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTc                      <  1:12564025/101‑1 (MQ=32)
                                                                                   aTCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTa                   >  1:14715523/1‑101 (MQ=33)
                                                                                   aTCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTa                   <  1:5728651/101‑1 (MQ=32)
                                                                                    tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTAt                  >  1:5529430/1‑101 (MQ=33)
                                                                                      gcgcCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTc                <  1:14672223/101‑1 (MQ=32)
                                                                                       cgcCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCa               >  1:8718828/1‑101 (MQ=33)
                                                                                       cgcCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCa               <  1:3791905/101‑1 (MQ=32)
                                                                                        gcCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCaa              <  1:13471466/101‑1 (MQ=32)
                                                                                         ccGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAAc             <  1:1415274/101‑1 (MQ=32)
                                                                                         ccGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTCGGCGTGGCCCGGAGCGTCGACGTGAGCGTAGTGACGCTTCTCGGTCTGGTACTCAAc             >  1:884783/1‑101 (MQ=2)
                                                                                         ccGTCCATCTGAGCAGCACCGGTGATCATGTTCTTGATGTAGTCGGCGTGGCCCGGAGCGTCGACGTGAGCGTAGTGACGCTTCTCGGTCTGGTACTCAAc             >  1:9121613/1‑101 (MQ=2)
                                                                                          cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACg            >  1:3323632/1‑101 (MQ=32)
                                                                                           gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGt           >  1:11031331/1‑101 (MQ=32)
                                                                                              cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTgag        <  1:11994412/101‑1 (MQ=32)
                                                                                                tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTGAGaa      >  1:7639029/1‑101 (MQ=32)
                                                                                                tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTGAGaa      <  1:2260285/101‑1 (MQ=32)
                                                                                                 cTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTGAGCAg     <  1:14063984/101‑1 (MQ=17)
                                                                                                  tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTGAGATgt    >  1:14169263/1‑101 (MQ=17)
                                                                                                  tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTGAGAAgt    >  1:8604365/1‑101 (MQ=32)
                                                                                                  tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTGAGAAgt    >  1:14419196/1‑101 (MQ=32)
                                                                                                   gcgcAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTGAGAAgtg   <  1:12850258/101‑1 (MQ=31)
                                                                                                    |                                                                                                    
TGTACGGAACGCCTACCTGACGACCCAGCAGGATGTGCTCACGAGTCTGCGGCATCGGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGGGTCGGGGTGTCGTATTCAACGTGAGAAGTGT  >  NC_007779/3460347‑3460547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.