Predicted mutation
evidence seq id position mutation freq annotation gene description
JC NC_007779 2,865,278 +TTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGC 28.9% coding (813/876 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 2865229 =138 (0.590)30 (0.240) 25/96 NT 28.9% coding (862/876 nt) rpoS RNA polymerase, sigma S (sigma 38) factor
?NC_007779 = 2865278 139 (0.590)coding (813/876 nt) rpoS RNA polymerase, sigma S (sigma 38) factor

TGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_007779/2865270‑2865229
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATC  <  NC_007779/2865278‑2865236
                                          ||||||||||||||||||||||||||||||||||||||||||                                           
TGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAA                            <  1:9294778/101‑1
 GCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAAT                           <  1:10580884/101‑1
  CGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATC                          >  1:2721112/1‑101
    CGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCT                        <  1:11095281/101‑1
    CGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCT                        >  1:1819714/1‑101
    CGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCT                        <  1:2751215/101‑1
     GAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTG                       <  1:3833019/101‑1
      AAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGC                      >  1:2903972/1‑101
       AATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCA                     <  1:9642171/101‑1
          CCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAAC                  <  1:9666307/101‑1
            TGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGC                >  1:11409787/1‑101
             GCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCA               <  1:1830222/101‑1
             GCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCA               <  1:5174795/101‑1
             GCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCA               >  1:5961858/1‑101
             GCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCA               <  1:9094221/101‑1
              CAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAG              >  1:9065179/1‑101
              CAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAG              >  1:8293591/1‑101
                AACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGG            <  1:3695079/101‑1
                 ACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGG           <  1:7067184/101‑1
                 ACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCAAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGG           >  1:6880281/1‑101
                  CGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGC          >  1:3155147/1‑101
                  CGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGC          <  1:1104741/101‑1
                   GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT         <  1:10700944/101‑1
                   GCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCT         <  1:6073722/101‑1
                    CAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTG        <  1:10942490/101‑1
                      GGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAA      <  1:2513312/101‑1
                       GGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAAT     <  1:2131317/101‑1
                        GGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATA    <  1:788725/101‑1
                         GCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATAT   <  1:3515196/101‑1
                          CTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATC  <  1:6078650/101‑1
                                          ||||||||||||||||||||||||||||||||||||||||||                                           
TGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_007779/2865270‑2865229
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATC  <  NC_007779/2865278‑2865236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.