Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0077791,472,0390G→A100.0% ‑0.5 1R323Q (CGG→CAG) insIIS30 transposase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/0):  ref base (1/0):  total (1/0)

CTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGA  >  NC_007779/1472021‑1472120
                  |                                                                                   
cTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAACCCCAAAGGGGCTATTCGGCCGGTACTTCCTAAAAAAGACATGCTTTCCCCATTTTCCCCAACTTGa  >  1:7368992/1‑101 (MQ=0)
                  |                                                                                   
CTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGA  >  NC_007779/1472021‑1472120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.