Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_007779	2,865,278	+TTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACAGCGCTTCGATATTCAGCCCCTGCGTTTGCAGGATTTCGCGCAAACGGCGC	10.1%	coding (813/876 nt)	rpoS ←	RNA polymerase, sigma S (sigma 38) factor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_007779	2865225 =	235 (0.890)	14 (0.090)	12/104	NT	10.1%	coding (866/876 nt)	rpoS	RNA polymerase, sigma S (sigma 38) factor
	?	NC_007779	= 2865278	195 (0.740)					coding (813/876 nt)	rpoS	RNA polymerase, sigma S (sigma 38) factor

TGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCTGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_007779/2865270‑2865225 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCG  <  NC_007779/2865278‑2865235                                               ||||||||||||||||||||||||||||||||||||||                                             TGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAA                             <  1:2088289/101‑1   CGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATC                           <  1:11077967/101‑1     CGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCT                         <  1:5849179/101‑1      aAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTG                        >  1:12766886/2‑101          TCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAA                    <  1:6747738/101‑1           CCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAAC                   <  1:6095434/101‑1             TGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGC                 >  1:10046320/1‑101              GCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCA                <  1:125997/101‑1               CAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAG               <  1:373695/101‑1                  ACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCGAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGG            >  1:12809621/1‑101                      AGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGA        >  1:4046988/1‑101                        GGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAAT      <  1:1074281/101‑1                        GGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAAT      <  1:2834140/101‑1                            TGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCG  <  1:1178128/101‑1                                               ||||||||||||||||||||||||||||||||||||||                                             TGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCGAAGCGCTGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_007779/2865270‑2865225 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCGCCGTTTGCGCGAAATCCTGCAAACGCAGGGGCTGAATATCG  <  NC_007779/2865278‑2865235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.