breseq  version 0.25c  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
JC 57,026 Δ21 bp 4.5% coding (64‑84/2355 nt) imp ← exported protein required for envelope biosynthesis and integrity
RA 66,457 C→A 100% V32F (GTT→TTT)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase
RA 97,880 C→T 100% S265F (TCC→TTC)  murD → UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
RA 167,632 C→A 100% P50Q (CCG→CAG)  fhuA → ferrichrome outer membrane transporter
RA 274,547 A→G 100% intergenic (‑206/‑2) insH ← / → mmuP IS5 transposase and trans‑activator/S‑methylmethionine transporter
MC JC 274,733 Δ97,240 bp 100% [mmuP][mhpD] 90 genes
RA 547,694 A→G 100% intergenic (+123/‑1156) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain/hypothetical protein
RA 547,831 +G 100% intergenic (+260/‑1019) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑binding Rossmann‑fold domain/hypothetical protein
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 569,996 G→C 100% intergenic (+345/‑120) ybcK → / → ybcL recombinase/kinase inhibitor
RA 749,354 C→A 100% P17P (CCG→CCT ybgO ← fimbrial‑like adhesin protein
RA 758,440 C→A 100% F110L (TTC→TTA sdhB → succinate dehydrogenase, FeS subunit
RA 887,234 C→A 100% A176A (GCG→GCT ybjJ ← transporter
RA 987,574 G→T 100% intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,093,686 T→C 100% V130A (GTA→GCA)  ycdT → diguanylate cyclase
RA 1,150,586 C→T 2.9% T84M (ACG→ATG)  fabH → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
RA 1,170,573 C→T 100% intergenic (‑164/‑77) ycfQ ← / → ycfR DNA‑binding transcriptional regulator/hypothetical protein
RA 1,213,001 T→C 2.4% intergenic (+245/+256) mcrA → / ← elbA 5‑methylcytosine‑specific restriction endonuclease B/hypothetical protein
RA 1,213,013 T→C 2.0% intergenic (+257/+244) mcrA → / ← elbA 5‑methylcytosine‑specific restriction endonuclease B/hypothetical protein
MC JC 1,301,041 Δ1,336 bp 100% insCinsD insC, insD
RA 1,318,876 G→A 100% P21S (CCT→TCT)  trpA ← tryptophan synthase subunit alpha
MC JC 1,413,639 Δ23,060 bp 100% [ydaO]ynaE 30 genes
RA 1,483,588 G→A 100% L322L (CTG→CTA ynbD → phosphatase, inner membrane protein
RA 1,496,775 Δ1 bp 100% coding (11/924 nt) ydcI ← DNA‑binding transcriptional regulator
RA 1,615,814 C→A 100% D202Y (GAC→TAC)  yneI ← aldehyde dehydrogenase
RA 1,669,599 Δ1 bp 100% coding (680/1221 nt) dgsA ← DNA‑binding transcriptional repressor
JC 1,724,327 Δ3 bp 9.9% coding (493‑495/2013 nt) ydhK → inner membrane protein
RA 1,844,073 G→A 100% intergenic (‑224/‑12) ynjH ← / → gdhA hypothetical protein/glutamate dehydrogenase, NADP‑specific
RA 1,918,872 T→A 100% S301T (TCC→ACC)  yebS → inner membrane protein
RA 1,973,476 G→A 100% L310L (CTC→CTT tar ← methyl‑accepting chemotaxis protein II
RA 2,005,401 C→T 100% E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,170,094 C→A 100% intergenic (‑437/+32) ogrK ← / ← yegR DNA‑binding transcriptional regulator/hypothetical protein
MC JC 2,320,795 Δ1,336 bp 100% insDinsC insD, insC
RA 2,406,075 C→T 100% V51I (GTT→ATT)  nuoF ← NADH:ubiquinone oxidoreductase, chain F
RA 2,416,102 G→A 100% L233L (CTG→TTG)  yfbS ← transporter
RA 2,538,628 C→T 100% T258T (ACC→ACT cysK → cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
RA 2,865,228 A→G 2.7% L288P (CTG→CCG)  rpoS ← RNA polymerase, sigma S (sigma 38) factor
JC 2,865,274 +46 bp 58.5% coding (817/876 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor
JC 2,865,278 +92 bp 37.6% coding (813/876 nt) rpoS ← RNA polymerase, sigma S (sigma 38) factor
RA 2,866,111 A→G 100% intergenic (‑21/+159) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/outer membrane lipoprotein
RA 3,003,330 G→A 100% L371L (CTC→CTT ygeV ← DNA‑binding transcriptional regulator
RA 3,049,815 G→T 100% R387S (CGC→AGC)  visC ← oxidoreductase
RA 3,397,813 C→G 100% D141H (GAT→CAT)  yhdE ← hypothetical protein
MC JC 3,742,936 Δ1,336 bp 100% insDinsC insD, insC
MC JC 3,746,911 Δ1,199 bp 100% insH insH
RA 3,750,122 Δ1 bp 97.0% coding (365/1416 nt) tnaA ← tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,750,123 C→T 3.0% G122S (GGC→AGC)  tnaA ← tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
MC JC 3,750,591 Δ1,199 bp 100% insH insH
RA 3,898,657 C→G 100% L259V (CTG→GTG)  yiaJ → DNA‑binding transcriptional repressor
MC JC 3,958,123 Δ34 bp 100% coding (1156‑1189/1287 nt) dctA → C4‑dicarboxylic acid, orotate and citrate transporter
RA 4,090,749 A→T 100% Q135L (CAG→CTG)  malQ → 4‑alpha‑glucanotransferase
RA 4,165,991 A→C 100% K43T (AAA→ACA)  rpsL → 30S ribosomal protein S12
MC JC 4,310,363 Δ1,199 bp 100% insH[alsK] insH, [alsK]
RA 4,347,517 T→C 22.2% L310P (CTG→CCG)  melA → alpha‑galactosidase
RA 4,371,271 Δ2 bp 100% intergenic (‑6/+299) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,546,535 T→A 100% intergenic (+296/‑182) fimB → / → fimE tyrosine recombinase/inversion of on/off regulator of fimA/tyrosine recombinase/inversion of on/off regulator of fimA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1288315–1288412 1288508–1288459 48–194 183 [181] [180] 184 ychS hypothetical protein
* * ÷ NC_007779 2176983–2178180 2178208 29–1226 323 [0] [0] 247 insH insH
* * ÷ NC_007779 3128828–3130002 3147473 17472–18646 183 [179] [0] 234 [yghO]–[yghZ] 19 genes
[yghO], insH, yghQ, yghR, yghS, yghT, pitB, gss, yghU, hybG, hybF, hybE, hybD, hybC, hybB, hybA, hybO, yghW, [yghZ]
* * ÷ NC_007779 3261134–3262332 3262337 6–1204 262 [0] [0] 286 insH–[tdcD] insH, [tdcD]

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 273179 =NA (NA)168 (0.700) 103/180 NT 63.9% coding (1109/1146 nt) ykfC hypothetical protein
?NC_007779 657877 = 95 (0.390)coding (164/210 nt) cspE DNA‑binding transcriptional repressor
* ? NC_007779 289856 =NA (NA)193 (1.270) 93/114 NT 100% intergenic (‑327/+17) argF/insB ornithine carbamoyltransferase 2, chain F/IS1 transposase InsAB'
?NC_007779 = 2176982 0 (0.000)intergenic (‑281/‑69) gatB/insH PTS system galactitol‑specific transporter subunit IIB/IS5 element protein
* ? NC_007779 = 290623NA (NA)6 (0.030) 6/176 NT 3.1% intergenic (‑53/‑101) insA/yagJ IS1 repressor protein InsA/hypothetical protein
?NC_007779 = 771566 189 (0.780)intergenic (+533/‑314) mngB/cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
* ? NC_007779 = 290625NA (NA)361 (1.500) 155/180 NT 57.7% intergenic (‑55/‑99) insA/yagJ IS1 repressor protein InsA/hypothetical protein
?NC_007779 678240 = 265 (1.100)coding (404/1428 nt) djlB chaperone
* ? NC_007779 380484 =NA (NA)221 (0.920) 124/180 NT NA intergenic (‑418/‑46) yaiO/insC hypothetical protein/IS2 insertion element repressor InsA
?NC_007779 = 381407 NA (NA)coding (510/906 nt) insD IS2 insertion element transposase InsAB'
* ? NC_007779 381401 =NA (NA)250 (1.060) 128/176 NT NA coding (504/906 nt) insD IS2 insertion element transposase InsAB'
?NC_007779 = 381812 NA (NA)coding (303/387 nt) yaiF hypothetical protein
* ? NC_007779 = 608570NA (NA)217 (0.930) 125/174 NT 89.3% intergenic (+170/+112) insL/entD IS186/IS421 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
?NC_007779 1305025 = 27 (0.110)coding (413/921 nt) oppB oligopeptide transporter subunit
* ? NC_007779 = 6542130 (0.000)216 (0.950) 117/170 NT 100% intergenic (+448/‑68) citB/insH DNA‑binding response regulator in two‑component regulatory system with citA/IS5 element protein
?NC_007779 655413 = 0 (0.000)intergenic (+152/‑1566) insH/crcA IS5 element protein/palmitoyl transferase for Lipid A
* ? NC_007779 = 657879NA (NA)147 (0.620) 90/174 NT NA coding (166/210 nt) cspE DNA‑binding transcriptional repressor
?NC_007779 = 1981404 NA (NA)intergenic (‑33/‑486) insH/yecG IS5 element protein/universal stress protein
* ? NC_007779 = 693388228 (0.950)337 (1.400) 144/180 NT 59.6% coding (452/1080 nt) ybeZ hypothetical protein
?NC_007779 = 1050967 NA (NA)intergenic (+15/+418) insB/cspH IS1 transposase InsAB'/stress protein, member of the CspA‑family
* ? NC_007779 771555 =205 (0.850)3 (0.010) 3/178 NT 1.5% intergenic (+522/‑325) mngB/cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
?NC_007779 = 1050966 NA (NA)intergenic (+14/+419) insB/cspH IS1 transposase InsAB'/stress protein, member of the CspA‑family
* ? NC_007779 1209367 =124 (0.510)89 (0.450) 65/148 NT 44.8% coding (290/630 nt) ycfK hypothetical protein
?NC_007779 1211196 = 117 (0.590)intergenic (‑500/‑66) tfaE/pin tail fiber assembly protein/site‑specific DNA recombinase
* ? NC_007779 = 1209382133 (0.550)73 (0.370) 55/148 NT 39.2% coding (305/630 nt) ycfK hypothetical protein
?NC_007779 = 1211179 117 (0.590)intergenic (‑483/‑83) tfaE/pin tail fiber assembly protein/site‑specific DNA recombinase
* ? NC_007779 1221489 =10 (0.040)210 (0.890) 113/176 NT 95.6% intergenic (+711/+2393) ymgF/ymgD hypothetical protein/hypothetical protein
?NC_007779 1301041 = NA (NA)intergenic (+219/‑89) ychE/insC inner membrane protein/IS2 element protein
* ? NC_007779 = 12214950 (0.000)199 (0.830) 112/180 NT 100% intergenic (+717/+2387) ymgF/ymgD hypothetical protein/hypothetical protein
?NC_007779 = 1302369 NA (NA)intergenic (+11/‑527) insD/oppA IS2 element protein/oligopeptide transporter subunit
* ? NC_007779 = 130503734 (0.140)231 (1.000) 119/172 NT 87.7% coding (425/921 nt) oppB oligopeptide transporter subunit
?NC_007779 2519723 = NA (NA)intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/transposase
* ? NC_007779 1397758 =NA (NA)198 (0.820) 111/180 NT 100% intergenic (‑122/‑32) ynaI/insH inner membrane protein/IS5 transposase and trans‑activator
?NC_007779 1672777 = 0 (0.000)intergenic (+111/‑313) ynfM/asr transporter/acid shock‑inducible periplasmic protein
* ? NC_007779 = 16727800 (0.000)213 (0.880) 112/180 NT 100% intergenic (+114/‑310) ynfM/asr transporter/acid shock‑inducible periplasmic protein
?NC_007779 1980209 = NA (NA)intergenic (‑289/+146) flhD/insH DNA‑binding transcriptional dual regulator/IS5 element protein
* ? NC_007779 1907280 =7 (0.030)205 (0.850) 111/180 NT 96.7% intergenic (+307/‑122) yobD/yebN inner membrane protein/inner membrane protein
?NC_007779 = 2412225 NA (NA)intergenic (‑55/‑782) insA/yfbQ IS1 element protein/aminotransferase
* ? NC_007779 = 190728811 (0.050)245 (1.020) 137/180 NT 95.7% intergenic (+315/‑114) yobD/yebN inner membrane protein/inner membrane protein
?NC_007779 2411458 = NA (NA)intergenic (‑147/+15) lrhA/insB DNA‑binding transcriptional repressor/IS1 element protein
* ? NC_007779 1980091 =0 (0.000)147 (0.610) 82/180 NT 100% intergenic (‑171/+264) flhD/insH DNA‑binding transcriptional dual regulator/IS5 element protein
?NC_007779 1980209 = NA (NA)intergenic (‑289/+146) flhD/insH DNA‑binding transcriptional dual regulator/IS5 element protein
* ? NC_007779 = 19800950 (0.000)232 (0.970) 131/178 NT 100% intergenic (‑175/+260) flhD/insH DNA‑binding transcriptional dual regulator/IS5 element protein
?NC_007779 2176984 = NA (NA)intergenic (‑283/‑67) gatB/insH PTS system galactitol‑specific transporter subunit IIB/IS5 element protein
* ? NC_007779 2178209 =0 (0.000)228 (0.950) 118/180 NT 100% intergenic (+178/+184) insH/gatZ IS5 element protein/D‑tagatose 1,6‑bisphosphate aldolase 2, subunit
?NC_007779 = 2412225 NA (NA)intergenic (‑55/‑782) insA/yfbQ IS1 element protein/aminotransferase
* ? NC_007779 2320794 =NA (NA)219 (0.910) 109/180 NT 100% intergenic (+633/+11) rcsB/insD DNA‑binding response regulator in two‑component regulatory system with RcsC and YojN/IS2 element protein
?NC_007779 = 2589834 0 (0.000)intergenic (+470/‑69) acrD/yffB aminoglycoside/multidrug efflux system/hypothetical protein
* ? NC_007779 = 2322124NA (NA)280 (1.160) 147/180 NT 100% intergenic (‑91/‑2589) insC/atoS IS2 element protein/sensory histidine kinase in two‑component regulatory system with AtoC
?NC_007779 2589830 = 0 (0.000)intergenic (+466/‑73) acrD/yffB aminoglycoside/multidrug efflux system/hypothetical protein
* ? NC_007779 2411458 =NA (NA)253 (1.050) 129/180 NT 95.1% intergenic (‑147/+15) lrhA/insB DNA‑binding transcriptional repressor/IS1 element protein
?NC_007779 = 2985055 13 (0.050)coding (553/1230 nt) yqeG transporter
* ? NC_007779 = 2412225NA (NA)253 (1.050) 136/180 NT 97.7% intergenic (‑55/‑782) insA/yfbQ IS1 element protein/aminotransferase
?NC_007779 2985047 = 6 (0.020)coding (545/1230 nt) yqeG transporter
* ? NC_007779 2941317 =290 (1.200)5 (0.020) 5/180 NT 1.7% intergenic (‑94/+257) gcvA/ygdI DNA‑binding transcriptional dual regulator/hypothetical protein
?NC_007779 = 3129996 NA (NA)intergenic (+146/+1) insH/yghQ IS5 transposase and trans‑activator/inner membrane protein
* ? NC_007779 = 2941321288 (1.200)7 (0.030) 7/180 NT 2.4% intergenic (‑98/+253) gcvA/ygdI DNA‑binding transcriptional dual regulator/hypothetical protein
?NC_007779 3128802 = NA (NA)coding (70/1173 nt) yghO DNA‑binding transcriptional regulator
* ? NC_007779 3147474 =0 (0.000)210 (0.870) 115/180 NT 100% coding (922/1041 nt) yghZ aldo‑keto reductase
?NC_007779 = 3262328 NA (NA)coding (1188/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic
* ? NC_007779 = 3186081NA (NA)247 (1.040) 129/178 NT 99.6% coding (26/2466 nt) yqiG outer membrane usher protein
?NC_007779 3512753 = 1 (0.000)coding (529/1026 nt) cytR DNA‑binding transcriptional dual regulator
* ? NC_007779 = 32611330 (0.000)238 (1.060) 124/168 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?NC_007779 = 3748109 NA (NA)intergenic (+150/+962) insH/tnaA IS5 element protein/tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_007779 3261134 =NA (NA)191 (0.790) 109/180 NT 100% intergenic (‑60/‑68) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?NC_007779 3265774 = 0 (0.000)coding (110/990 nt) tdcB catabolic threonine dehydratase, PLP‑dependent
* ? NC_007779 3261135 =NA (NA)245 (1.030) 130/178 NT 100% intergenic (‑61/‑67) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?NC_007779 3262338 = 0 (0.000)coding (1178/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic
* ? NC_007779 = 32657770 (0.000)266 (1.100) 141/180 NT 100% coding (107/990 nt) tdcB catabolic threonine dehydratase, PLP‑dependent
?NC_007779 3365411 = NA (NA)intergenic (+368/+146) yhcD/insH outer membrane protein/IS5 transposase and trans‑activator
* ? NC_007779 = 35127591 (0.000)217 (0.910) 119/178 NT 99.5% coding (535/1026 nt) cytR DNA‑binding transcriptional dual regulator
?NC_007779 = 3744262 NA (NA)intergenic (‑90/+31) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
* ? NC_007779 = 3744263NA (NA)246 (1.020) 115/180 NT 99.6% intergenic (‑91/+30) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
?NC_007779 3903274 = 1 (0.000)coding (37/825 nt) bax hypothetical protein
* ? NC_007779 3746911 =NA (NA)253 (1.050) 119/180 NT 100% intergenic (‑509/‑68) mdtL/insH multidrug efflux system protein/IS5 element protein
?NC_007779 = 3748646 0 (0.000)intergenic (+687/+425) insH/tnaA IS5 element protein/tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_007779 = 3748105NA (NA)263 (1.090) 135/180 NT 100% intergenic (+146/+966) insH/tnaA IS5 element protein/tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
?NC_007779 3748643 = 0 (0.000)intergenic (+684/+428) insH/tnaA IS5 element protein/tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_007779 = 39032796 (0.020)241 (1.010) 130/178 NT 97.6% coding (42/825 nt) bax hypothetical protein
?NC_007779 = 4504190 NA (NA)intergenic (+10/‑89) insD/yjgW IS2 insertion element transposase InsAB'/hypothetical protein
* ? NC_007779 3987186 =NA (NA)283 (1.190) 139/178 NT 100% intergenic (‑732/‑31) slp/insH outer membrane lipoprotein/IS5 transposase and trans‑activator
?NC_007779 = 4009203 0 (0.000)coding (1379/1623 nt) yhiJ hypothetical protein
* ? NC_007779 = 3988379NA (NA)256 (1.060) 126/180 NT 100% coding (746/783 nt) yhiS hypothetical protein
?NC_007779 4009199 = 0 (0.000)coding (1375/1623 nt) yhiJ hypothetical protein
* ? NC_007779 4310363 =NA (NA)263 (1.090) 128/180 NT 100% intergenic (‑287/‑68) yjcS/insH alkyl sulfatase/IS5 element protein
?NC_007779 4353540 = 0 (0.000)intergenic (‑118/+453) dcuB/dcuR C4‑dicarboxylate antiporter/DNA‑binding response regulator in two‑component regulatory system with DcuS
* ? NC_007779 = 4311557NA (NA)227 (0.940) 127/180 NT 100% coding (921/1029 nt) alsK D‑allose kinase
?NC_007779 = 4353543 0 (0.000)intergenic (‑121/+450) dcuB/dcuR C4‑dicarboxylate antiporter/DNA‑binding response regulator in two‑component regulatory system with DcuS
* ? NC_007779 4355060 =0 (0.000)277 (1.150) 132/180 NT 100% coding (1281/1632 nt) dcuS sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
?NC_007779 4502861 = NA (NA)intergenic (+241/‑46) intB/insC integrase/IS2 insertion element repressor InsA
* ? NC_007779 = 43550662 (0.010)183 (0.780) 119/176 NT 98.9% coding (1275/1632 nt) dcuS sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
?NC_007779 = 4504189 NA (NA)intergenic (+9/‑90) insD/yjgW IS2 insertion element transposase InsAB'/hypothetical protein